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Merck
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Key Documents

SRP3299

Sigma-Aldrich

GDF-5 mouse

recombinant, expressed in E. coli, ≥98% (SDS-PAGE), ≥98% (HPLC)

Sinónimos:

BMP-14, CDMP-1

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About This Item

Código UNSPSC:
12352202
NACRES:
NA.32

origen biológico

mouse

recombinante

expressed in E. coli

Análisis

≥98% (HPLC)
≥98% (SDS-PAGE)

formulario

lyophilized

potencia

0.08-0.12 μg/mL

mol peso

27.0 kDa

envase

pkg of 50 μg

impurezas

<0.1 EU/μg endotoxin, tested

color

white to off-white

Nº de acceso UniProt

P43027

Condiciones de envío

wet ice

temp. de almacenamiento

−20°C

Información sobre el gen

mouse ... GDF5(14563)

Descripción general

Mutations in the GDF-5 gene have been implicated in Grebe Syndrome, which is characterized by short stature, extra digits, short and deformed extremities, and in Hunter-Thompson type dwarfism. The mature and functional form of GDF-5 is a homodimer of two 120 amino-acid polypeptide chain (monomers) linked by a single disulfide bond. Each GDF-5 monomer is expressed as the C-terminal part of a precursor polypeptide, which also contains a 27 amino-acid signal peptide and a 348 amino-acid propeptide. This precursor undergoes intracellular dimerization, and upon secretion it is processed by a furin-type protease.

Acciones bioquímicas o fisiológicas

GDF-5 is expressed in long bones during embryonic development and postnatally in articular cartilage. Recombinant murine GDF-5 is a 27.0 kDa homodimeric disulfide-linked protein consisting of two 120 amino acids.

Forma física

Lyophilized with no additives.

Reconstitución

Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/mL. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.

Código de clase de almacenamiento

10 - Combustible liquids

Clase de riesgo para el agua (WGK)

WGK 3

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable

Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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Eiichi Hinoi et al.
Biochemical and biophysical research communications, 450(1), 255-260 (2014-06-20)
We have previously demonstrated promotion by growth differentiation factor-5 (GDF5) of brown adipogenesis for systemic energy expenditure through a mechanism relevant to activating the bone morphological protein (BMP) receptor/mothers against decapentaplegic homolog (Smad)/peroxisome proliferator-activated receptor gamma co-activator 1α (PGC-1α) pathway.
Cartilage-derived morphogenetic proteins. New members of the transforming growth factor-beta superfamily predominantly expressed in long bones during human embryonic development.
Chang, C.H. et al.
The Journal of Biological Chemistry, 45, 28227-28234 (1994)
Zhou Pei et al.
Archives of biochemistry and biophysics, 560, 27-35 (2014-08-01)
Adipocyte differentiation is key to determining the number of adipocytes during the development of obesity. Recent studies have shown that growth differentiation factor-5 (GDF5) promotes brown adipogenesis, however its role in white adipogenesis is still uncertain. The aim of the

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