SRP3144

PLGF-3 human

recombinant, expressed in E. coli, ≥95% (SDS-PAGE), ≥95% (HPLC), suitable for cell culture

• Sinónimos: PGFL, Placenta Growth Factor-3
• Código UNSPSC: 12352202

Propiedades

• origen biológico: human
• recombinante: expressed in E. coli
• Análisis: ≥95% (HPLC); ≥95% (SDS-PAGE)
• formulario: lyophilized
• potencia: 5.0-50.0 ng/mL
• mol peso: 45.7 kDa
• envase: pkg of 25 μg
• técnicas: cell culture | mammalian: suitable
• impurezas: <0.1 EU/μg endotoxin, tested
• color: white
• Nº de acceso UniProt: P49763
• Condiciones de envío: wet ice
• temp. de almacenamiento: −20°C
• Información sobre el gen: human ... PGF(5228)

Descripción

Descripción general

PLGF (placental growth factor), originally identified in human placenta, is a member of the VEGF (vascular endothelial growth factor) family. This protein is predominantly expressed in placenta, and also shows expression in heart, thyroid, lung and skeletal muscle. It is alternatively spliced to produce four isoforms in humans namely, PLGF1, PLGF2, PLGF3 and PLGF4. PLGF gene is localized to human chromosome 14q24, spans 800kb and is composed of seven exons.
Recombinant human PlGF-3 is a 45.7kDa disulfide-linked homodimeric protein of two 203 amino acid polypeptide chains.

Acciones bioquímicas o fisiológicas

PLGF (placental growth factor) is involved in angiogenesis, but does not play an essential role in angiogenesis during reproduction and development. However, inactivation of this gene in pathological conditions such as, ischemia, inflammation and cancer, results in impaired angiogenesis. Preclinical studies show that this protein might be a therapeutic target for treatment of angiogenesis and inflammation related disorders. In acute coronary syndrome (ACS) patients, increased levels of PLGF are linked with obstructive sleep apnea (OSA) and short-term adverse outcomes.

Secuencia

LPAVPPQQWA LSAGNGSSEV EVVPFQEVWG RSYCRALERL VDVVSEYPSE VEHMFSPSCV SLLRCTGCCG DENLHCVPVE TANVTMQLLK IRSGDRPSYV ELTFSQHVRC ECRHSPGRQS PDMPGDFRAD APSFLPPRRS LPMLFRMEWG CALTGSQSAV WPSSPVPEEI PRMHPGRNGK KQQRKPLREK MKPERCGDAV PRR

Forma física

Lyophilized with no additives.

Reconstitución

Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.

Información de seguridad

• Código de clase de almacenamiento: 11 - Combustible Solids
• Clase de riesgo para el agua (WGK): WGK 3
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable