SRP3143
Pleiotrophin human
recombinant, expressed in E. coli, ≥98% (SDS-PAGE), ≥98% (HPLC), suitable for cell culture
Sinónimos:
Heparin Affin Regulatory Protein (HARP), Heparin-binding growth factor-8(HBGF-8), Osteoblast-Specific Factor-1 (OSF-1), PTN
Iniciar sesiónpara Ver la Fijación de precios por contrato y de la organización
About This Item
Código UNSPSC:
12352202
NACRES:
NA.32
Productos recomendados
origen biológico
human
recombinante
expressed in E. coli
Ensayo
≥98% (HPLC)
≥98% (SDS-PAGE)
Formulario
lyophilized
mol peso
15.4 kDa
envase
pkg of 20 μg
técnicas
cell culture | mammalian: suitable
impurezas
<0.1 EU/μg endotoxin, tested
color
white
Nº de acceso UniProt
Condiciones de envío
wet ice
temp. de almacenamiento
−20°C
Información sobre el gen
human ... PTN(5764)
Descripción general
PTN (pleiotrophin) is a 136-residue, platelet-derived growth factor (PDGF)-inducible, heparin-binding cytokine. It shares ~50% similarity with midkine, a retinoic acid-inducible factor. It is expressed in a tissue and stage dependent manner during development, but in adults, this protein is expressed constitutively, though in a restricted tissue pattern. PTN is a low molecular weight protein composed of two domains linked by disulfide bridges, with three antiparallel β-sheets in each domain. This gene is localized to human chromosome 7q33-q34. Recombinant human Pleiotrophin is a 15.4 kDa protein containing 136 amino acid residues and five intra-molecular disulfide bonds.
Acciones bioquímicas o fisiológicas
PTN (pleiotrophin) is a ligand for receptor protein tyrosine phosphatase (RPTP) β/ζ, and binds with it and inhibits its catalytic capabilities in a “ligand-dependent receptor inactivation”-mechanism, and promotes tyrosine phosphorylation of β-catenin. It acts as a mitogen for fibroblasts, endothelial cells, epithelial cells, and trophoblasts. It promotes neurite outgrowth and glial process outgrowth from neurons and glial progenitor cells, respectively. This protein functions as a protooncogene, and is up-regulated in multiple types of tumors, where it promotes tumor angiogenesis and progression. This protein functions as a crucial mitogen during nerve, bone, vascular, and tumor growth, and has been found to be up-regulated in macrodactyly, identifying it as a candidate gene for macrodactyly.
Secuencia
GKKEKPEKKV KKSDCGEWQW SVCVPTSGDC GLGTREGTRT GAECKQTMKT QRCKIPCNWK KQFGAECKYQ FQAWGECDLN TALKTRTGSL KRALHNAECQ KTVTISKPCG KLTKPKPQAE SKKKKKEGKK QEKMLD
Forma física
Lyophilized from 10 mM sodium phosphate, pH 7.5.
Reconstitución
Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.
Palabra de señalización
Warning
Frases de peligro
Consejos de prudencia
Clasificaciones de peligro
Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3
Órganos de actuación
Respiratory system
Código de clase de almacenamiento
13 - Non Combustible Solids
Clase de riesgo para el agua (WGK)
WGK 3
Punto de inflamabilidad (°F)
Not applicable
Punto de inflamabilidad (°C)
Not applicable
Elija entre una de las versiones más recientes:
Certificados de análisis (COA)
Lot/Batch Number
¿No ve la versión correcta?
Si necesita una versión concreta, puede buscar un certificado específico por el número de lote.
¿Ya tiene este producto?
Encuentre la documentación para los productos que ha comprado recientemente en la Biblioteca de documentos.
Pleiotrophin signals increased tyrosine phosphorylation of beta beta-catenin through inactivation of the intrinsic catalytic activity of the receptor-type protein tyrosine phosphatase beta/zeta.
Meng K
Proceedings of the National Academy of Sciences of the USA, 97, 2603-2608 (2000)
Midkine and pleiotrophin in neural development and cancer
Kadomatsu K and Muramatsu T
Cancer Letters, 204, 127-143 (2004)
T Muramatsu
The International journal of developmental biology, 37(1), 183-188 (1993-03-01)
Using mouse teratocarcinoma system, we found a novel retinoic acid responsive gene. Midkine (MK), the product of the gene is a secreted, heparin-binding protein of molecular weight 14,000. MK gene is intensely expressed in the midgestation period, and in the
Takashi Muramatsu
Journal of biochemistry, 132(3), 359-371 (2002-09-03)
Midkine (MK) and pleiotrophin (PTN) are low molecular weight proteins with closely related structures. They are mainly composed of two domains held by disulfide bridges, and there are three antiparallel beta-sheets in each domain. MK and PTN promote the growth
Thomas F Deuel et al.
Archives of biochemistry and biophysics, 397(2), 162-171 (2002-02-14)
Pleiotrophin (PTN the protein, Ptn the gene) is a 136 amino acid secreted heparin-binding cytokine that signals diverse functions, including lineage-specific differentiation of glial progenitor cells, neurite outgrowth, and angiogenesis. Pleiotrophin gene expression is found in cells in early differentiation
Nuestro equipo de científicos tiene experiencia en todas las áreas de investigación: Ciencias de la vida, Ciencia de los materiales, Síntesis química, Cromatografía, Analítica y muchas otras.
Póngase en contacto con el Servicio técnico