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Key Documents

SRP3112

Sigma-Aldrich

MEC (CCL28) human

recombinant, expressed in E. coli, ≥98% (SDS-PAGE), ≥98% (HPLC), suitable for cell culture

Sinónimos:

CCK-1, CCL28, Mucosae-associated Epithelial Chemokine

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About This Item

Código UNSPSC:
12352202
NACRES:
NA.32

origen biológico

human

recombinante

expressed in E. coli

Análisis

≥98% (HPLC)
≥98% (SDS-PAGE)

formulario

lyophilized

mol peso

12.3 kDa

envase

pkg of 20 μg

técnicas

cell culture | mammalian: suitable

impurezas

<0.1 EU/μg endotoxin, tested

color

white to off-white

Nº de acceso UniProt

Condiciones de envío

wet ice

temp. de almacenamiento

−20°C

Información sobre el gen

human ... CCL28(56477)

Descripción general

MEC is a secreted CC chemokine expressed primarily by epithelial cells of the bronchioles, salivary gland, mammary gland and colon. MEC signals through the CCR10 receptor and chemoattracts resting CD4, CD8 T-cells and eosinophils. MEC contains six cysteines including the four highly conserved cysteine residues present in CC chemokines. Recombinant human MEC is a 12.3 kDa protein containing 108 amino acid residues.

Acciones bioquímicas o fisiológicas

MEC is a secreted CC chemokine expressed primarily by epithelial cells of the bronchioles, salivary gland, mammary gland and colon. Recombinant human MEC is a 12.3 kDa protein containing 108 amino acid residues.

Secuencia

SEAILPIASS CCTEVSHHIS RRLLERVNMC RIQRADGDCD LAAVILHVKR RRICVSPHNH TVKQWMKVQA AKKNGKGNVC HRKKHHGKRN SNRAHQGKHE TYGHKTPY

Forma física

Lyophilized with no additives.

Reconstitución

Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.

Código de clase de almacenamiento

11 - Combustible Solids

Clase de riesgo para el agua (WGK)

WGK 3

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable


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J Pan et al.
Journal of immunology (Baltimore, Md. : 1950), 165(6), 2943-2949 (2000-09-07)
Mucosae-associated epithelial chemokine (MEC) is a novel chemokine whose mRNA is most abundant in salivary gland, with strong expression in other mucosal sites, including colon, trachea, and mammary gland. MEC is constitutively expressed by epithelial cells; MEC mRNA is detected
Eric J Kunkel et al.
Immunity, 16(1), 1-4 (2002-02-05)
Tissue-selective trafficking of memory and effector T and B lymphocytes is mediated by unique combinations of adhesion molecules and chemokines. The discovery of several related epithelial-expressed chemokines (TECK/CCL25 in small intestine, CTACK/CCL27 in skin, and MEC/CCL28 in diverse mucosal sites)
Emese Zsiros et al.
Clinical cancer research : an official journal of the American Association for Cancer Research, 21(12), 2840-2850 (2015-02-26)
Chemokines are implicated in T-cell trafficking. We mapped the chemokine landscape in advanced stage ovarian cancer and characterized the expression of cognate receptors in autologous dendritic cell (DC)-vaccine primed T cells in the context of cell-based immunotherapy. The expression of

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