SRP0213

PTP1B (1-321)/PTPN1 Active from mouse

recombinant, expressed in E. coli, ≥90% (SDS-PAGE)

• Sinónimos: PTPN1, Protein tyrosine phosphatase, non-receptor type 1
• Código UNSPSC: 12352200
• NACRES: NA.32

Propiedades

• origen biológico: mouse
• recombinante: expressed in E. coli
• Análisis: ≥90% (SDS-PAGE)
• formulario: aqueous solution
• mol peso: 63.3 kDa
• envase: pkg of 20 μg
• condiciones de almacenamiento: avoid repeated freeze/thaw cycles
• concentración: >0.02 mg/mL
• Nº de acceso NCBI: NM_011201.3
• Nº de acceso UniProt: P35821
• Condiciones de envío: dry ice
• temp. de almacenamiento: −70°C
• Información sobre el gen: human ... PTPN1(5770)

Descripción

Descripción general

Protein tyrosine phosphatase, Catalytic domain, (amino acids 1-321), with N-terminal GST tag, MW = 63.3 kDa, expressed in an E. coli expression system.

Aplicación

Useful for the study of enzyme kinetics, regulation, to dephosphorylate target substrates and for screening inhibitors.

Definición de unidad

One unit will hydrolyze 1 nmol p-nitrophenyl phosphate per minute at pH 7.4 and 30°C.

Forma física

Formulation: recombinant proteins were eluted in 25 mM Tris-HCL, 75 mM NaCl, pH 8.0, 0.05% Tween, 10 mM glutathione, 1 mM DTT and 50% glycerol.

Nota de preparación

Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.