SRP0162

JMJD3/KDM6B Active human

recombinant, expressed in baculovirus infected insect cells, ≥70% (SDS-PAGE)

• Sinónimos: JmjC domain-containing protein 3, KDM6B, KIAA0346, lysine (K)-specific demethylase 6B
• Código UNSPSC: 12352200
• NACRES: NA.32

Propiedades

• origen biológico: human
• recombinante: expressed in baculovirus infected insect cells
• Ensayo: ≥70% (SDS-PAGE)
• Formulario: aqueous solution
• mol peso: 71 kDa
• envase: pkg of 20 μg
• Nº de acceso NCBI: NM_001080424
• Nº de acceso UniProt: O15054
• Condiciones de envío: dry ice
• temp. de almacenamiento: −70°C
• Información sobre el gen: human ... KDM6B(23135)

Descripción

Descripción general

Human JMJD3/KDM6B histone demethylase (amino acids 1043-end), Genbank Accession No. NM_001080424, with C-terminal FLAG-tag, MW = 71 kDa, expressed in Sf9 cells via a baculovirus expression system.

KDM6B (lysine demethylase 6B)/ JMJD3 (jumonji domain-containing 3) is a major histone demethylase protein. This protein contains a JmjC domain in its C-terminal.

Aplicación

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Acciones bioquímicas o fisiológicas

KDM6B (lysine demethylase 6B) is responsible for the demethylation of histone H3K27. At promoters it demethylates the repressive H3K27me3 and therefore, promotes gene expression. In human and mouse fibroblasts, it demethyates the H3K27me3 at INK4a/ARF (alternate reading frame) locus, thus, promoting the expression of p16 protein, and therefore, acts as a tumor suppressor. It is also involved in inflammatory pathways in macrophages and embryonic stem cell differentiation. The expression of KDM6B is elevated in clear cell renal cell carcinoma (ccRCC) where it promotes EMT (epithelial to mesenchymal transition). Increased expression is also linked positively with lymph node metastasis, tumor size, cancer stage and poor prognosis.

Forma física

Formulated in 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 10% glycerol and 3 mM DTT.

Nota de preparación

Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.

Información de seguridad

• Código de clase de almacenamiento: 12 - Non Combustible Liquids
• Clase de riesgo para el agua (WGK): WGK 1
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable