SRP0132

EZH2 / EED / SUZ12 human

recombinant, expressed in baculovirus infected insect cells, ≥90% (SDS-PAGE)

• Sinónimos: ENX-1, EZH2, Enhancer of zeste homolog 2, KMT6A, Lysine N-methyltransferase 6A
• Código UNSPSC: 12352200
• NACRES: NA.32

Propiedades

• origen biológico: human
• recombinante: expressed in baculovirus infected insect cells
• Análisis: ≥90% (SDS-PAGE)
• formulario: aqueous solution
• mol peso: 51 kDa (EED); 86 kDa (EZH2); 87 kDa (SUZ12)
• envase: pkg of 50 μg
• concentración: >0.02 mg/mL
• Nº de acceso NCBI: NM_003797 (EED); NM_004456 (EZH2); NM_015355 (SUZ12)
• Nº de acceso UniProt: Q15910
• Condiciones de envío: dry ice
• temp. de almacenamiento: −70°C
• Información sobre el gen: human ... EZH2(2146)

Descripción

Descripción general

Complex of: Human EZH2 (GenBank Accession No. NM_004456), (amino acids 2-end) with N-terminal His tag, MW = 86 kDa. Human EED (GenBank Accession No. NM_003797) (amino acid 2-end) with N-terminal Flag tag, MW = 51 kDa. Human SUZ12, NM_015355, amino acids 2-end, with N-terminal His tag.

Aplicación

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Definición de unidad

One unit is defined as the amount of enzyme required to methylate 1 pmol of substrate/min at 37°C.

Forma física

Formulated in 25 mM Tris-HCl, pH 8.0, 138 mM NaCl, 0.05% Tween-20, 100 μg/ml flag peptide and 10% glycerol.

Nota de preparación

Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.