Saltar al contenido
Merck

SH1931

Sigma-Aldrich

MISSION® shRNA Human Gene Family Set, Lentiviral Particles

Apoptosis Pathway

Iniciar sesiónpara Ver la Fijación de precios por contrato y de la organización


About This Item

Código UNSPSC:
41106609
NACRES:
NA.51

En este momento no podemos mostrarle ni los precios ni la disponibilidad

Nivel de calidad

Línea del producto

MISSION®

temp. de almacenamiento

−70°C

Información sobre el gen

¿Está buscando productos similares? Visita Guía de comparación de productos

Comparar elementos similares

Ver comparación completa

Mostrar Diferencias

1 of 4

Este artículo
4996350553900205
assay

≥98% β-phase basis (unsintered powder)

assay

≥98% β-phase basis (sintered Powder)

assay

-

assay

-

Quality Level

200

Quality Level

200

Quality Level

100

Quality Level

100

grade

puriss. p.a.

grade

puriss. p.a.

grade

-

grade

-

anion traces

chloride (Cl-): ≤100 mg/L

anion traces

chloride (Cl-): ≤50 mg/kg

anion traces

-

anion traces

-

impurities

≤250 mg/kg total sulfur (as SO4) (ICP), ≤50 mg/kg total heavy metals (as lead)

impurities

≤50 mg/kg total heavy metals as lead, ≤500 mg/kg total sulfur as SO4 (ICP)

impurities

-

impurities

-

Descripción general

Lentiviral particles (representational, plate titer) are provided in 96-well plates that are barcoded for simple identification. Aliquots of 4 x 50μl are provided to prevent the need for excessive freeze/thaw, and maximize functional viral particles. Each gene family set is representationally titered (10% of clones). Fully titered sets are also available, for more information inquire at RNAi@sial.com. A CD containing RefSeq, gene description, gene symbol, clone ID, hairpin sequence, locus link, and plate map positions are provided with the gene family set.

Otras notas

Each MISSION shRNA clone is constructed within the lentivirus plasmid vector, pLKO.1-Puro, followed by transformation into Escherichia coli. The pLKO.1-Puro vector contains bacterial (ampicillin) and mammalian (puromycin) antibiotic resistance genes for selection of inserts in either bacterial or mammalian cell lines. Each clone set consists of an average of 3-5 constructs that have been designed against each target gene using a proprietary algorithm. Therefore, a range of knockdown efficiency, with at least one construct from each gene set being >70%, can be expected when using these clones. This allows one to examine the effect of loss of gene function over a large series of gene knockdown efficiencies. Each shRNA construct has been cloned and sequence verified to ensure a match to the target gene.
For a detailed listing of other available gene family sets, visit the gene family set website.
Number of Genes: 443, Number of Clones: 3512
The exact gene and clone count at time of purchase may vary slightly as the TRC library is continually updated.

Información legal

Use of this product is subject to one or more license agreements. For details, please see http://sigmaaldrich.com/missionlicense .
MISSION is a registered trademark of Merck KGaA, Darmstadt, Germany

Código de clase de almacenamiento

12 - Non Combustible Liquids

Clase de riesgo para el agua (WGK)

WGK 3

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable

Equipo de protección personal

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)


  • Certificados de análisis (COA)

    Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

    ¿Necesita un COA de muestra?

    Esto es un Certificado de análisis (COA) de muestra y es posible que no represente un lote de fabricación reciente para este producto específico.

    ¿Ya tiene este producto?

    Encuentre la documentación para los productos que ha comprado recientemente en la Biblioteca de documentos.

    Visite la Librería de documentos

    Role of apoptosis in the pathogenesis of COPD and pulmonary emphysema
    Demedts, I. K., et al.
    Respirology, 7, 53-53 (2006)
    Apoptosis, pyroptosis, and necrosis: mechanistic description of dead and dying eukaryotic cells.
    Susan L Fink et al.
    Infection and immunity, 73(4), 1907-1916 (2005-03-24)
    Susan E Logue et al.
    Biochemical Society transactions, 36(Pt 1), 1-9 (2008-01-23)
    Apoptosis, a highly controlled mode of cell death, is utilized to eliminate superfluous, aged, injured or infected cells from the body. Caspases, a family of aspartic acid-specific proteases, are the major effectors of apoptosis. To curtail their activity, caspases are
    Dmitri V Krysko et al.
    Methods (San Diego, Calif.), 44(3), 205-221 (2008-03-04)
    Three major morphologies of cell death have been described: apoptosis (type I), cell death associated with autophagy (type II) and necrosis (type III). Apoptosis and cell death associated with autophagy can be distinguished by certain biochemical events. However, necrosis is
    J C Burns et al.
    Proceedings of the National Academy of Sciences of the United States of America, 90(17), 8033-8037 (1993-09-01)
    The restricted host-cell range and low titer of retroviral vectors limit their use for stable gene transfer in eukaryotic cells. To overcome these limitations, we have produced murine leukemia virus-derived vectors in which the retroviral envelope glycoprotein has been completely

    Nuestro equipo de científicos tiene experiencia en todas las áreas de investigación: Ciencias de la vida, Ciencia de los materiales, Síntesis química, Cromatografía, Analítica y muchas otras.

    Póngase en contacto con el Servicio técnico