SAE0215

Inorganic Pyrophosphatase from Escherichia coli

≥100 units/mL, buffered aqueous solution

• Código UNSPSC: 12352204

Propiedades

• origen biológico: Escherichia coli
• Nivel de calidad: 200
• recombinante: expressed in E. coli
• grado: Molecular Biology; for molecular biology
• descripción: Recombinant, expressed in E.coli
• Ensayo: ≥95% (size exclusion chromatography)
• Formulario: buffered aqueous solution
• actividad específica: ≥100 units/mL
• caducidad: 2 yr at -20 °C ((retest))
• mol peso: 19.7 kDa
• condiciones de almacenamiento: OK to freeze
• concentración: ≥100 units/mL
• color: colorless
• pH óptimo: 9.0 (25 °C)
• pH: 8.0 (25 °C)
• solubilidad: soluble; water: miscible
• idoneidad: suitable for molecular biology
• Nº de acceso UniProt: P0A7A9
• aplicaciones: research use
• actividad extraña: DNAse, none detected; RNAse, none detected; Nickase, none detected
• Condiciones de envío: dry ice
• temp. de almacenamiento: -10 to -25°C

Descripción

Descripción general

Inorganic pyrophosphatase (PPase) is a ubiquitous enzyme that catalyzes pyrophosphate hydrolysis. It plays an important role in energy metabolism by providing a thermodynamic pull for biosynthetic reactions, such as protein, RNA, and DNA synthesis. Nucleic acid synthesis would be energetically impossible in vivo if not coupled with the hydrolysis of pyrophosphate (PPi).

Aplicación

This product is based on the native pyrophosphatase from E. coli, Uniprot No. P0A7A9. Pyrophosphatase in E. coli is a homohexameric protein containing 175 amino acids residues per subunit. This product is a recombinant protein expressed in E. coli and induced by IPTG. Each subunit has a MW of 19.7 kDa and theoretical pI of ~5. The protein activity is Mg2+ dependent and it is a relatively thermostable protein.

Acciones bioquímicas o fisiológicas

Inorganic pyrophosphatase (PPase) is a ubiquitous enzyme that catalyzes pyrophosphate to phosphate. It plays an important role in energy metabolism as it provides a thermodynamic pull for biosynthetic reactions, such as protein, RNA, and DNA synthesis.

Características y beneficios

This product has a purity minimum of 95% (SEC-HPLC) and an activity minimum of 100 units/mL to enhance RNA yield during transcription.

Definición de unidad

One unit will release 1.0 µmole of inorganic orthophosphate per minute at pH 9 at 25 °C. The reaction buffer used for determination of enzyme activity contains 50 mM Tris-HCl, pH 9.0.

Forma física

The product is supplied as an aqueous solution containing 20mM Tris-HCl, 100mM NaCl, 1mM DTT, 0.1mM EDTA, and 50% glycerol, titrated to pH 8 at 25 °C.

Información de seguridad

• Código de clase de almacenamiento: 10 - Combustible liquids
• Clase de riesgo para el agua (WGK): WGK 1