SAE0111

Phosphatase, Alkaline from human placenta

≥10 units/mg solid (in glycine buffer)

• Sinónimos: Phosphatase, Alkaline from human placenta, Orthophosphoric-monoester phosphohydrolase (alkaline optimum)
• Número de CAS: 9001-78-9
• Comisión internacional de enzimas: 3.1.3.1 (BRENDA, IUBMB)
• Número MDL: MFCD00131849
• Código UNSPSC: 12352200
• NACRES: NA.54

Propiedades

• origen biológico: human placenta
• actividad específica: ≥10 units/mg solid (in glycine buffer)
• Nº de acceso UniProt: P05187
• temp. de almacenamiento: −20°C
• Información sobre el gen: human ... ALPP(250)

Descripción

Aplicación

Alkaline phosphatase is used for conjugation to antibodies and other proteins for ELISA, Western blotting, and histochemical detection. It is routinely used to dephosphorylate proteins and nucleic acids. It may be used for protein labeling when high sensitivity is required. Alkaline phosphatase may be also be used to dephosphorylate the 5′-termini of DNA or RNA to prevent self-ligation. DNA or RNA can also be tagged with radiolabeled phosphate (via T4 polynucleotide kinase) after dephosphorylation with alkaline phosphatase.

Acciones bioquímicas o fisiológicas

Alkaline phosphatase dephosphorylates proteins and nucleic acids. Human placental alkaline phosphatase (PLAP) has an unique residue, Glu-429. It is an allosteric enzyme that is uncompetitively inhibited by L-Phe, L-Trp, and L-Leu. PLAP is post-translationally modified. Phosphatidylinositol-specific phospholipase C may be involved in the release of PLAP. PLAP may have a role in cell division and may be involved in the transfer of maternal IgG to the fetus.

Definición de unidad

One unit will hydrolyze 1.0 umole of P-Nitrophenyl Phosphate per minute at pH 10.4 at 37 Deg C.

Información de seguridad

• Pictogramas: GHS08
• Palabra de señalización: Danger
• Frases de peligro: H334
• Consejos de prudencia: P261 - P284 - P501
• Clasificaciones de peligro: Resp. Sens. 1
• Código de clase de almacenamiento: 11 - Combustible Solids
• Clase de riesgo para el agua (WGK): WGK 1
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable