SAE0044
Choline Oxidase from Arthrobacter sp.
recombinant, expressed in E. coli ≥12 units/mg protein
Sinónimos:
COD, COX, ChOx, Choline: oxygen 1-oxidoreductase
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About This Item
Productos recomendados
origen biológico
bacterial (arthrobacter sp.)
Nivel de calidad
recombinante
expressed in E. coli ≥12 units/mg protein
formulario
powder
actividad específica
≥12 units/mg protein
técnicas
activity assay: suitable
Nº de acceso UniProt
temp. de almacenamiento
−20°C
Descripción general
Research area: Cell Signaling
Choline oxidase from Arthrobacter sp. is a flavoprotein, which is a member of the glucose–methanol–choline (GMC). The codA gene encodes the choline oxidase enzyme.
Choline oxidase from Arthrobacter sp. is a flavoprotein, which is a member of the glucose–methanol–choline (GMC). The codA gene encodes the choline oxidase enzyme.
Aplicación
Choline oxidase from Arthrobacter sp. has been used in the enzymatic determination of choline in milk using a flow injection analysis (FIA) system with potentiometric detection. It has been used for fluorescent detection of choline released during autotaxin (ATX) activity in conditioned media and plasma.
Acciones bioquímicas o fisiológicas
Choline oxidase from Arthrobacter sp. is a flavoprotein, which is a member of the GMC-oxidoreductase family. The enzyme catalyzes the four-electron-oxidation of choline to glycine betaine via the intermediate betaine aldehyde, in two sequential FAD-dependent reaction steps. The enzyme is useful for enzymatic determination of phosphatidylcholine by coupling with phospholipase D or determination of sphingomyelin by coupling with sphingomyelinase and for cholinesterase activity assays.Choline oxidase (COD) mediating glycine betaine biosynthetic pathway plays a vital role in creating stress resistant transgenic plants.
Definición de unidad
One unit will form 1 μmole of H2O2 with oxidation of 1 μmole of choline to betaine aldehyde per min at pH 8.0 at 37 °C. Note: During the conversion of choline to betaine by choline oxidase, 2 μmoles of H2O2 are produced for every μmole of choline.
Nota de preparación
It is recommended to reconstitute choline oxidase in phosphate buffer at pH 7.5 supplemented with 10mM EDTA. Reconstituted material can be stored at 2-8 °C for at least 2 weeks.
Almacenamiento y estabilidad
Tightly closed.Dry. Keep locked up or in an area accessible only to qualified or authorized
persons
persons
Palabra de señalización
Danger
Frases de peligro
Consejos de prudencia
Clasificaciones de peligro
Resp. Sens. 1
Código de clase de almacenamiento
11 - Combustible Solids
Clase de riesgo para el agua (WGK)
WGK 3
Punto de inflamabilidad (°F)
Not applicable
Punto de inflamabilidad (°C)
Not applicable
Certificados de análisis (COA)
Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»
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Los clientes también vieron
Transformation of Synechococcus with a gene for choline oxidase enhances tolerance to salt stress.
Deshnium P, et al.
Plant Molecular Biology, 29(5), 897-907 (1995)
New enzymatic assay of cholinesterase activity
Okabe, H. et al.,
Clinica Chimica Acta; International Journal of Clinical Chemistry, 80, 87-94 (1977)
Rapid, specific, and sensitive measurements of plasma sphingomyelin and phosphatidylcholine.
Hojjati M.R., and Jiang X.C.
Journal of Lipid Research, 47, 673-676 (2006)
Transformation of Arabidopsis thaliana with the codA gene for choline oxidase; accumulation of glycinebetaine and enhanced tolerance to salt and cold stress.
Hayashi H, et al.
The Plant Journal, 12(1), 133-142 (1997)
J L Lima et al.
The Analyst, 125(7), 1281-1284 (2000-09-14)
The development of a FIA system for the determination of total choline content in several types of milk is described. The samples were submitted to hydrochloric acid digestion before injection into the system and passed through an enzymatic reactor containing
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