SAB5900201

Alpaca Anti-GFP, recombinant single domain Antibody

Recombinant Alpaca(VHH) Nano antibody

• Código UNSPSC: 12352203

Propiedades

• origen biológico: alpaca
• Nivel de calidad: 100
• recombinante: expressed in (E. coli)
• conjugado: unconjugated
• forma del anticuerpo: (Affinity purified VHH antibody)
• clon: GE10, monoclonal
• Análisis: >95% (SDS-PAGE)
• formulario: lyophilized solid
• mol peso: 13.7 kDa; ~15-18 kDa (SDS PAGE under reducing condition)
• purificado por: (Affinity chromatography purified via Ni-charged resin); affinity chromatography (purified via Ni-charged resin)
• concentración: 1 mg/mL
• técnicas: ELISA: 1:2000-1:10000; immunofluorescence: 1:100-1:600; immunoprecipitation (IP): 0.5-4.0 μg; western blot: 1:1000-1:5000
• color: colorless
• pH: ( in PBS, pH 7.4)
• isotipo: VHH
• Nº de acceso Protein ID: BAG71039.1
• Nº de acceso UniProt: A0A059PIQ0
• Condiciones de envío: ambient
• temp. de almacenamiento: 2-8°C
• modificación del objetivo postraduccional: unmodified

Descripción

Descripción general

Green fluorescent protein (GFP) is a protein composed of 238 amino acid residues(26.9kDa) derived from the Jellyfish Aequorea victoria, which emits green light (emission peak at 509nm) when excited by blue light (excitation peak at 395nm). GFP has become an invaluable tool in cell biology research, since its intrinsic fluorescence can be visualized in living cells. EGFP contains the double-amino-acid substitutions Phe-64 to Leu and Ser-65 to Thr (previously published as GFPmut1; PMID: 8707053). In contrast to wtGFP, EGFP has a single, strong, red-shifted excitation peak at 488 nm

Especificidad

Green fluorescence protein (GFP) and other derivatives of GFP

Inmunógeno

Recombinant full length GFP protein

Aplicación

ELISA, WB, IP, IF

Envase

50 µg per vial / 100 µg per vial

Forma física

Purified VHH protein in buffer containing 25 mM TAPS pH8.5, 500 mM NaCl, 5 mM EDTA

Reconstitución

Reconstitute the lyophilized protein with sterile deionized water or with 50% glycerol (ACS grade or better) to a stock solution of 1 mg/mL. Solubilize for 30 to 60 minutes at room temperature with occasional gentle mixing. Avoid vigorous shaking or vortexing.

Almacenamiento y estabilidad

Stable for 1 years at 4°C from date of receipt.

Otras notas

Storage at 4°C; Do not freeze.

Información de seguridad

• Código de clase de almacenamiento: 12 - Non Combustible Liquids
• Clase de riesgo para el agua (WGK): WGK 1
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable