SAB5700590

Recombinant 2019-nCoV Spike RBD Protein with His tag

• Sinónimos: 2019-nCoV RBD Protein, NCP-CoV RBD Protein, S glycoprotein Subunit1 RBD Protein, S1-RBD protein, novel coronavirus RBD Protein
• Código UNSPSC: 12352203
• NACRES: NA.26

Propiedades

• recombinante: expressed in HEK 293 cells
• Nivel de calidad: 100
• Ensayo: >95% (HPLC); >95% (SDS-PAGE)
• Formulario: lyophilized powder
• mol peso: 25.9 kDa
• impurezas: <0.1 EU/μg protein (LAL test)
• Nº de acceso UniProt: P0DTC2
• Condiciones de envío: wet ice
• temp. de almacenamiento: −20°C
• Información sobre el gen: SARS virus ... S(43740568)

Descripción

Descripción general

Recombinant 2019-nCoV Spike RBD-His Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Arg319-Phe541) of 2019-ncov Spike RBD-His (Accession #YP_009724390.1) fused with a 6XHis tag at the C-terminus.

Acciones bioquímicas o fisiológicas

Measured by its binding ability in a functional ELISA. Immobilized Recombinant SARS-COV-2 Spike RBD-His at 2μg/mL (100 μL/well) can bind Recombinant Human ACE2 with a linear range of 0.15-4.53 ng/mL. Immobilized human ACE2 on COOH Chip, can bind SARS-COV-2 Spike RBD Protein with an affinity constant of 36.7 nM as determined in a SPR assay.

Reconstitución

For lyophilized protein : Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water.

Almacenamiento y estabilidad

Store the lyophilized protein at -20°C to -80°C for long term. After reconstitution, the protein solution is stable at -20°C for 3 months, at 2-8 °C for up to 1 week. This product is stable at ≤ -70°C for up to 6 months from the date of receipt. For optimal storage, aliquot into smaller quantities after centrifugation and store at recommended temperature.

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Data presented is the available current product information and provided as-is. These products have not been tested or verified in any additional applications, sample types, including any clinical use. Experimental conditions must be empirically derived by the user. Our Antibody Guarantee only covers tested applications stated herein and conditions presented in our product information and is not extended to publications.

Información de seguridad

• Código de clase de almacenamiento: 11 - Combustible Solids
• Clase de riesgo para el agua (WGK): WGK 3
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable