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Key Documents

SAB5200010

Sigma-Aldrich

Monoclonal Anti-DNA/RNA Damage antibody produced in mouse

clone 15A3, 1 mg/mL, purified immunoglobulin

Sinónimos:

Anti-8 hydroxy guanine, Anti-8-OH-dG, Anti-8OHG, Anti-DNA/RNA Damage

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About This Item

Código UNSPSC:
12352203
NACRES:
NA.41

origen biológico

mouse

Nivel de calidad

conjugado

unconjugated

forma del anticuerpo

purified immunoglobulin

tipo de anticuerpo

primary antibodies

clon

15A3, monoclonal

formulario

buffered aqueous glycerol solution

concentración

1 mg/mL

técnicas

immunohistochemistry: 1:1000
immunoprecipitation (IP): suitable
indirect ELISA: suitable

isotipo

IgG2b

Condiciones de envío

wet ice

temp. de almacenamiento

−20°C

modificación del objetivo postraduccional

unmodified

Categorías relacionadas

Descripción general

DNA/RNA damage, also known as 8-hydroxyguanine (8-OHG), is a product of oxidized DNA or RNA.

Especificidad

Recognizes markers of oxidative damage to DNA (8-hydroxy-2′-deoxyguanosine, 8-hydroxyguanine and 8-hydroxyguanosine).

Inmunógeno

8-hydroxy-guanosine-BSA and casein conjugates

Acciones bioquímicas o fisiológicas

8-hydroxyguanine (8-OHG) helps in determining the reactive oxygen species (ROS)-induced modification of a purine residue in DNA. It is a sensitive marker of oxidative DNA damage. Overexpression of the gene has been observed in diabetic patients, especially those with advanced microvascular complications. Thus, 8-OHG functions as a novel biomarker for identifying oxidative DNA damage in diabetic patients. Plasma level of 8-OHG elevates with the age, with cigarette smoking and during tumorigenesis. 8-OHG levels in urine is considered as a biomarker of the total systemic oxidative stress in vivo. Overexpression of 8-OHG has been observed in various neurodegenerative diseases such as Parkinson′s disease (PD) and multiple system atrophy (MSA).
DNA or RNA damage is due to environmental factors and normal metabolic processes inside the cell, that then hinder the ability of the cell to carry out its functions. There are four main types of DNA due to endogenous cellular processes and they are oxidation, alkylation, hydrolysis and mismatch of the bases. During the oxidation of bases, highly reactive chemical entities collectively known as RONS, occurs. RONS stands for reactive oxygen and nitrogen species and includes nitric oxide, superoxide, hydroxyl radical, hydrogen peroxide and peroxynitrite. Numerous studies have shown that RONS causes a variety of issues including DNA damage. 8-hydroxyguanine, 8-hydroxy-2′-eoxyguanonsine and 8- hydroxyguanosine are all RNA and DNA markers of oxidative damage. 8-hydroxy-2′-guanosine is produced by reactive oxygen and nitrogen species including hydroxyl radical and peroxynitrite. Specically its high biological relevance is due to its ability to induce G to T transversions, which is one of the most frequent somatic mutations. 8-hydroxy-guanine has been the most frequently studied type of DNA base damage, with studies in diabetes, and cancer. Base modications of this type arise from radical-induced hydroxylation and cleavage reactions of the purine ring. And nally, 8-hydroxy-guanosine, like 8-hydroxy-2′-guanosine, induces a mutagenic transversion of G to T in DNA. Its role has specically been tested in the development of diabetes, hypertension and strokes.

Características y beneficios

Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more.

Forma física

Solution in PBS, 50% glycerol, and 0.09% sodium azide

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Código de clase de almacenamiento

10 - Combustible liquids

Clase de riesgo para el agua (WGK)

WGK 1

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable


Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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Serum 8-Hydroxy-Guanine Levels Are Increased in Diabetic Patients
Shin CS
Diabetes Care, 24, 733-737 (2001)
Systemic Increase of Oxidative Nucleic Acid Damage in Parkinson's Disease and Multiple System Atrophy
Kikuchi A
Neurobiology of Disease, 9, 244-248 (2002)
Yi Yang et al.
Chemical biology & drug design, 99(6), 884-896 (2022-03-22)
Alzheimer's disease (AD) is a chronic neurodegenerative disorder that can cause cognitive impairment. Ginsenoside Rg1 (Rg1) has a significant neuroprotective effect on animals with memory impairment. However, the mechanism of how Rg1 mediates the Wnt signaling pathway and improves cognitive

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