SAB4200870

Anti-Akkermancia muciniphila antibody produced in rabbit

IgG fraction of antiserum

• Código UNSPSC: 12352203
• NACRES: NA.41

Propiedades

• origen biológico: rabbit
• Nivel de calidad: 200
• forma del anticuerpo: IgG fraction of antiserum
• tipo de anticuerpo: primary antibodies
• Formulario: liquid
• reactividad de especies: (Akkermancia muciniphila)
• envase: pkg of 100 μL; pkg of 25 μL
• concentración: ~1 mg/mL
• técnicas: immunoblotting: 1:500-1:1000 using Akkermansia muciniphila lysate
• Condiciones de envío: dry ice
• temp. de almacenamiento: −20°C
• modificación del objetivo postraduccional: unmodified

Descripción

Descripción general

Akkermansia muciniphila is a Gram negative, oval shaped, non-motile, non-spore forming strictly anaerobic bacteria.1 A. muciniphila colonized in the mucus layer of the human intestine niche. A muciniphila inhabits the gastrointestinal tracts of most of healthy adults and represents 1-4% of the total fecal microbiota starting from early life.2 It is one of the top 20 most abundant species detectable in the human gut.3 It was found that A. muciniphila abundance in the gut was correlated to a healthy intestine and inversely correlated to many disease conditions.2 In comparison to healthy controls, A. muciniphila levels were low in intestinal disorders, such inflammatory bowel disease IBD, but also in other conditions, such as autism, atopy, and obesity.2,5-8 Therefore, A. muciniphila was suggested to serve as a marker of healthy intestine.
In addition, goring research identified A. muciniphila as a promising potential probiotic that can be administrated for the treatment of diseases such as, colitis, metabolic syndromes, immune diseases and cancer. However more research is needed to verify the safety of oral administration of A. muciniphila in humans.3

Especificidad

Anti-Akkermansia muciniphila antibody recognizes A. muciniphila lysate and whole dead bacteria.

Acciones bioquímicas o fisiológicas

A. muciniphila is able to degrade mucin, a key mucus component, using the enzymes sialidase and fucosidase, and utilize it as a source of carbon and nitrogen.2 Mucin degradation by A. muciniphila results in the production of short-chain fatty acids (SCFA), that become available metabolites to the host and also can stimulate the host immune system. Moreover, SCFA promotes the growth and metabolic activity of mucus- associated gut microbiota preventing the pathogenic bacteria from reaching the intestinal cells.4

Forma física

Supplied as a solution in 0.01 M phosphate buffered saline pH 7.4, containing 15 mM sodium azide as a preservative.

Almacenamiento y estabilidad

For continuous use, store at 2-8°C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog  our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Información de seguridad

• Código de clase de almacenamiento: 12 - Non Combustible Liquids
• Clase de riesgo para el agua (WGK): nwg
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable