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Merck

SAB4200805

Sigma-Aldrich

Anti-β-Galactosidase antibody, Mouse monoclonal

clone GAL-13, purified from hybridoma cell culture

Sinónimos:

Anti-Beta-gal, Anti-Beta-galactosidase, Anti-Lactase

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About This Item

Código UNSPSC:
12352203
NACRES:
NA.41

origen biológico

mouse

forma del anticuerpo

purified from hybridoma cell culture

tipo de anticuerpo

primary antibodies

clon

GAL-13, monoclonal

descripción

Research area: Microbiome

Formulario

buffered aqueous solution

reactividad de especies

E. coli

envase

antibody small pack of 25 μL

concentración

~1.0 mg/mL

técnicas

immunofluorescence: suitable
immunohistochemistry: suitable
indirect ELISA: 0.5-1 μg/mL using anti-Mouse antibody for coating, primary Anti-Beta-GAL antibody followed by addition of β-d-galactosidase enzyme from E. coli and 2-Nitrophenyl β-D-galactopyranoside as substrate.

isotipo

IgG1

Condiciones de envío

dry ice

temp. de almacenamiento

−20°C

modificación del objetivo postraduccional

unmodified

Información sobre el gen

Escherichia coli K12 ... Lacz(945006)

Descripción general

Anti-nitric oxide synthase, inducible antibody, mouse monoclonal (mouse IgG1 isotype) is derived from the NOS-IN hybridoma, produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mouse immunized with a synthetic peptide corresponding to the C terminal region of nitric oxide synthase (NOS) of mouse macrophage origin (iNOS, also termed macNOS), conjugated to KLH
Beta galactosidases have been obtained from microorganisms such as fungi, bacteria and yeasts; plants, animals cells and from recombinant sources. This enzyme has two main applications; the removal of lactose from milk products for lactose intolerant people and the production of galactosylated products.Monoclonal Anti-Beta-Galactosidase recognizes soluble Beta-d-galactosidase and retains the enzymatic activity. The antibody reacts with the native enzyme and does not recognize denatured or reduced Beta-galactosidase. The Anti-Beta-Galactosidase antibody maybe useful tool for immunoenzymatic staining of blood and bone marrow smears or tissue sections. In addition, this product may be used as a primary antibody for the detection and purification of recombinant fusion hybrid proteins, which contain Beta-galactosidase (lacZ) in the cloning vector.

Inmunógeno

Beta-D-Galactosidase purified from E. coli

Aplicación

Anti-Nitric Oxide Synthase, Inducible antibody, Mouse monoclonal has been used in: immunoblotting immunofluorescence fluorescence-activated cell sorting 3 (FACS3) immunohistochemistry
The antibody is recommended to use in various immunological techniques, including ELISA, Immunofluorescence and immunohistology.

Acciones bioquímicas o fisiológicas

NO is a messenger molecule mediating diverse functions including vasodilatation, neurotransmission, antimicrobial and anti-tumor activities. It is implicated as a pathogenic mediator in a variety of conditions, including central nervous system (CNS) disease states, such as the animal model of multiple sclerosis (MS) and experimental allergic encephalomyelitis.

Forma física

Supplied as a solution in 0.01 M phosphate buffered saline pH 7.4, containing 15 mM sodium azide as a preservative.

Otras notas

This product is for R&D use only, not for drug, household, or other uses.

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Código de clase de almacenamiento

10 - Combustible liquids

Clase de riesgo para el agua (WGK)

WGK 1

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable


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Erythropoietin-?-D-galactosidase the generation, purification and use of a fusion protein
Nielsen, O.J. et al.,
Journal of Immunological Methods, 111, 1-9 (1988)
Nitric oxide release: Part II
Carpenter AW and Mark HS
Chemical Society Reviews, 41(10), 3742-3752 (2012)
Neuronal nitric oxide synthase: structure, subcellular localization, regulation, and clinical implications
Zhou L and Zhu DY
Nitric Oxide, 20(4), 223-230 (2009)
Anjali Bajpai et al.
Developmental biology, 457(1), 1-8 (2019-09-27)
The Hedgehog (Hh) morphogen regulates growth and patterning. Since Hh signaling is also implicated in carcinogenesis, it is conceivable that de novo Hh-secreting organizers, if formed in association with oncogenic hit could be tumor-cooperative. Here we validate this hypothesis using
S Gillard-Blaas et al.
Journal of immunological methods, 139(2), 207-215 (1991-06-03)
A T cell hybridoma specific for a protein of Plasmodium chabaudi chabaudi has been used to test a system for direct T cell screening of a cDNA library of P. chabaudi in the phage lambda gt11. The technique is based

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