SAB4200716

Anti-Vimentin antibody, Mouse monoclonal

clone VIM-13.2, purified from hybridoma cell culture

• Sinónimos: Anti-F5H288
• Código UNSPSC: 12352203
• NACRES: NA.41

Propiedades

• origen biológico: mouse
• forma del anticuerpo: purified from hybridoma cell culture
• tipo de anticuerpo: primary antibodies
• clon: VIM-13.2, monoclonal
• formulario: buffered aqueous solution
• mol peso: ~58 kDa
• reactividad de especies: mouse, bovine, canine, rat, monkey, human, chicken
• validación mejorada: independent; Learn more about Antibody Enhanced Validation
• concentración: ~1.0 mg/mL
• técnicas: immunoblotting: 0.25-0.5 μg/mL using human foreskin fibroblast HS68 cell line extract; immunofluorescence: 1.25-2.5 μg/mL using human foreskin fibroblast HS68 cells; immunohistochemistry: suitable
• isotipo: IgM
• Condiciones de envío: dry ice
• temp. de almacenamiento: −20°C
• modificación del objetivo postraduccional: unmodified
• Información sobre el gen: bovine ... Vim(280955) ; chicken ... Vim(420519) ; dog ... Vim(477991) ; human ... VIM(7431) ; mouse ... Vim(22352) ; rat ... Vim(81818) ; rhesus monkey ... Vim(705289)

Descripción

Descripción general

Anti-Vimentin antibody, Mouse monoclonal (mouse IgM isotype) is derived from the VIM-13.2 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from a BALB/c mouse immunized with human foreskin fibroblast extract. Vimentin is a type III intermediate filaments (IF) expressed in a wide range of cell types. including, pancreatic precursor cells, fibroblasts and sertoli cells. It is also expressed in trophoblastic giant cells, neuronal precursor cells, lung epithelial cells, renal tubular and stromal cells. Vimentin is also present in leucocytes, macrophages, neutrophils and mesangial cells.

Inmunógeno

human foreskin fibroblast extract

Aplicación

Anti-Vimentin antibody, Mouse monoclonal has been used in:

• western blotting
• indirect immunofluorescence
• immunohistochemistry

Acciones bioquímicas o fisiológicas

Vimentin plays a significant role in supporting and anchoring the cellular position of organelles, such as nucleus, endoplasmic reticulum, mitochondria (either laterally or terminally). It helps to maintain the mesenchymal cells especially their shape, motility, survival during of mechanical stress. Epithelial cancers like prostate, breast, lung show an increased vimentin expression. Vimentin is also highly expressed in gastrointestinal tumors, central nervous system tumors and malignant melanoma.

Forma física

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Información de seguridad

• Código de clase de almacenamiento: 12 - Non Combustible Liquids
• Clase de riesgo para el agua (WGK): nwg
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable