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Merck

SAB4200631

Sigma-Aldrich

Monoclonal Anti-CYBA antibody produced in mouse

clone CB-64, purified from hybridoma cell culture

Sinónimos:

Cytochrome b558 subunit alpha, Neutrophil cytochrome b 22 kDa polypeptide, Superoxide-generating NADPH oxidase light chain subunit, cytochrome b-245 alpha polypeptide, p22-PHOX

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About This Item

Código UNSPSC:
12352203
NACRES:
NA.41

origen biológico

mouse

Nivel de calidad

forma del anticuerpo

purified immunoglobulin

tipo de anticuerpo

primary antibodies

clon

CB-64, monoclonal

formulario

buffered aqueous solution

reactividad de especies

bovine, mouse, rat, monkey, human, canine

concentración

~1 mg/mL

técnicas

flow cytometry: 5-10 μg/test using HeLa cells.
immunoblotting: 0.5-1 μg/mL using whole extracts of HeLa cells.
immunofluorescence: 5-10 μg/mL using HeLa cells.

isotipo

IgM

Nº de acceso UniProt

Condiciones de envío

dry ice

temp. de almacenamiento

−20°C

modificación del objetivo postraduccional

unmodified

Información sobre el gen

human ... CYBA(1535)
mouse ... Cyba(13057)
rat ... Cyba(79129)

Descripción general

Monoclonal Anti-CYBA (mouse IgM isotype) is derived from the hybridoma CB-64 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a synthetic peptide corresponding to a sequence at the internal region of human CYBA, conjugated to keyhole limpet hemocyanin (KLH). The small subunit, p22phox subunit, also known as cytochrome b245, alpha polypeptide/light chain (CYBA) is membrane bound,which is expressed in phagocytic, endothelial and vascular smooth muscle cells. This gene is located on human chromosome16q24

Inmunógeno

Synthetic peptide corresponding to a sequence at the internal region of human CYBA .

Aplicación

Monoclonal Anti-CYBA antibody produced in mouse has been used in:
  • immunoblotting
  • flow cytometry
  • immunofluorescence

Acciones bioquímicas o fisiológicas

In prostate cancer cells downregulation of cytochrome b245, alpha polypeptide/light chain (CYBA) was reported to inhibited cell proliferation and colony formation, through AKT and ERK1/2 signaling pathways, indicating CYBA has a role in tumor angiogenesis and tumor growth. Mutations in this gene causes an autosomal recessive forms of chronic granulomatous disease (AR220CGD).

Forma física

Supplied as a solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide as a preservative.

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Código de clase de almacenamiento

10 - Combustible liquids

Clase de riesgo para el agua (WGK)

WGK 1

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable


Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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Visite la Librería de documentos

Qi Li et al.
Biochimica et biophysica acta, 1833(12), 3375-3385 (2013-10-12)
Excessive generation of reactive oxygen species (ROS) in cancer cells is associated with cancer development, but the underlying mechanisms and therapeutic significance remain elusive. In this study, we reported that levels of ROS and p22(phox) expression are greatly increased in
CYBA encoding p22phox, the cytochrome b558 alpha polypeptide: gene structure, expression, role and physiopathology
Stasia MJ
Gene, 586(1) (2016)
Is the C242T Polymorphism of the CYBA Gene Linked with Oxidative Stress-Associated Complications of Prematurity
Huizing MJ, et al.
Antioxidants & Redox Signaling, 27(17) (2017)
Gorka San José et al.
Clinical science (London, England : 1979), 114(3), 173-182 (2008-01-11)
Oxidative stress plays a key role in the pathophysiology of several major cardiovascular diseases, including atherosclerosis, hypertension, heart failure, stroke and diabetes. ROS (reactive oxygen species) affect multiple tissues either directly or through NO depletion. ROS induce cardiovascular dysfunction by
NADPH oxidase subunit p22phox-mediated reactive oxygen species contribute to angiogenesis and tumor growth through AKT and ERK1/2 signaling pathways in prostate cancer
Li Q, et al.
Biochimica et Biophysica Acta - Molecular Cell Research, 1833(12) (2013)

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