SAB4200597

Monoclonal Anti-FMR1 antibody produced in mouse

clone FMR2.5, hybridoma cell culture supernatant

• Sinónimos: FMRP, FRAXA, POF, POF1, fragile X mental retardation 1
• Código UNSPSC: 12352203
• NACRES: NA.41

Propiedades

• origen biológico: mouse
• forma del anticuerpo: purified immunoglobulin
• tipo de anticuerpo: primary antibodies
• clon: FMR2.5, monoclonal
• formulario: buffered aqueous solution
• mol peso: ~71 kDa
• reactividad de especies: rat, human, mouse, hamster
• concentración: ~1 mg/mL
• técnicas: flow cytometry: 2.0-5.0 μg/test using HeLa cells; immunoblotting: 1.0-2.0 μg/mL using HepG2 total cell extracts.; immunocytochemistry: suitable; immunofluorescence: 5-10 μg/mL using HepG2 cells.
• isotipo: IgG1
• Nº de acceso UniProt: Q06787
• Condiciones de envío: dry ice
• temp. de almacenamiento: −20°C
• modificación del objetivo postraduccional: unmodified
• Información sobre el gen: human ... FMR1(2332) ; mouse ... Fmr1(14265) ; rat ... Fmr1(24948)

Descripción

Descripción general

Monoclonal Anti-FMR1 (mouse IgG1 isotype) is derived from the hybridoma FMR2.5 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a synthetic peptide. Fragile X mental retardation 1 (FMR1) is encoded by the gene mapped to human chromosome Xq27.3q28. FMR1 localizes to both the nucleus and the cytoplasm.

Especificidad

Monoclonal Anti- FMR1 recognizes human, hamster, rat and mouse FMR1.

Inmunógeno

synthetic peptide corresponding to a sequence at the C-terminal region of human FMR1

Aplicación

Monoclonal Anti-FMR1 antibody produced in mouse may be used in:

• immunoblotting
• immunocytochemistry
• immunofluorescence
• flow cytometry

Acciones bioquímicas o fisiológicas

Fragile X mental retardation 1 (FMR1) protein functions as an RNA-binding protein that associates with polyribosomes and is a likely component of a messenger ribonuclear protein (mRNP) particle. Since, FMR1 contains both a nuclear localization signal and a nuclear export signal it is also implicated in nucleocytoplasmic transport. Mutation in the gene leads to the development of fragile X mental retardation (FXMR) syndrome.

Forma física

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Almacenamiento y estabilidad

For extended storage, freeze at 20 °C in working aliquots. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Información de seguridad

• Código de clase de almacenamiento: 10 - Combustible liquids
• Clase de riesgo para el agua (WGK): nwg
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable