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Merck

SAB4200545

Sigma-Aldrich

Anti-Parvalbumin antibody, Mouse monoclonal

clone PARV-19, purified from hybridoma cell culture

Sinónimos:

Monoclonal Anti-ALPHA-PARVALBUMIN, Monoclonal Anti-PALB1, Monoclonal Anti-PV, Monoclonal Anti-PVALB (includes EG:19293), Monoclonal Anti-Parv, Monoclonal Anti-Pva

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About This Item

Código UNSPSC:
12352203
NACRES:
NA.44

origen biológico

mouse

Nivel de calidad

conjugado

unconjugated

forma del anticuerpo

purified from hybridoma cell culture

tipo de anticuerpo

primary antibodies

clon

PARV-19, monoclonal

formulario

buffered aqueous solution

mol peso

antigen 12 kDa

reactividad de especies

rat, human

concentración

~1.0 mg/mL

técnicas

immunohistochemistry: 5-10 μg/mL using formalin-fixed paraffin embedded rat cerebellum.
indirect immunofluorescence: 10-20 μg/mL using BLO-11 cells.
western blot: 1-2 μg/mL using mouse muscle extracts.

isotipo

IgG1

Condiciones de envío

dry ice

temp. de almacenamiento

−20°C

modificación del objetivo postraduccional

unmodified

Información sobre el gen

human ... PVALB(5816)
rat ... PVALB(25269)

Descripción general

Monoclonal Anti-Parvalbumin (mouse IgG1 isotype) is derived from the hybridoma PARV-19 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with purified frog muscle parvalbumin. Parvalbumin (PV) is a member of the family of Ca2+-binding molecules. It carries three helix-loop-helix EF-hand Ca2+ binding motifs, with the two C-terminal motifs.

Especificidad

Monoclonal Anti-Parvalbumin recognizes parvalbumin in a Ca2+ -ion dependent manner

Inmunógeno

purified frog muscle parvalbumin. The isotype is determined by ELISA using Mouse Monoclonal Antibody Isotyping Reagents (Sigma ISO-2).

Aplicación

Anti-Parvalbumin antibody, Mouse monoclonal has been used in:
  • immunohistochemistry
  • immunofluorescence staining
  • immunolabeling
  • to label brain slices for perineuronal net staining
  • enzyme-linked immunosorbent assay (ELISA)
  • immunocytochemistry

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Western Blotting (1 paper)

Acciones bioquímicas o fisiológicas

Ca2+ participates in cell motility, release of neurotransmitters, membrane permeability, excitation−contraction of muscles, cell secretory procedures, and transmission of nerve impulses. Intracellular Ca2+ exchangers are essential to modulate these processes efficiently. Parvalbumin (PV), a calcium-binding protein, can regulate the intracellular calcium dynamics in neurons. Hence it may have an impact on facilitation and depression at synapses. Interestingly, PV deficiency alters neuronal activity, a key mechanism leading to epileptic seizures or Parkinson.

Forma física

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Almacenamiento y estabilidad

For extended storage, freeze at –20 °C in working aliquots. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Código de clase de almacenamiento

10 - Combustible liquids

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable


Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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BioEssays : news and reviews in molecular, cellular and developmental biology, 31(4), 410-421 (2009-03-11)
Parvalbumins (PVs) are acidic, intracellular Ca(2+)-binding proteins of low molecular weight. They are associated with several Ca(2+)-mediated cellular activities and physiological processes. It has been suggested that PV might function as a "Ca2+ shuttle" transporting Ca2+ from troponin-C (TnC) to
Rachel Schroeder et al.
Antioxidants & redox signaling, 35(7), 511-530 (2021-01-28)
Aims: Impaired embryonic cortical interneuron development from prenatal stress is linked to adult neuropsychiatric impairment, stemming in part from excessive generation of reactive oxygen species in the developing embryo. Unfortunately, there are no preventive medicines that mitigate the risk of
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Developmental neurobiology, 76(10), 1078-1091 (2016-01-03)
Prenatal stress is associated with altered behavioral, cognitive, and psychiatric outcomes in offspring. Due to the importance of GABAergic systems in normal development and in psychiatric disorders, prenatal stress effects on these neurons have been investigated in animal models. Prenatal
Angela E Gonzalez et al.
Frontiers in cellular neuroscience, 16, 932391-932391 (2022-08-16)
Parvalbumin (PV)-positive cells are GABAergic fast-spiking interneurons that modulate the activity of pyramidal neurons in the medial prefrontal cortex (mPFC) and their output to brain areas associated with learning and memory. The majority of PV cells within the mPFC are
Emily T Jorgensen et al.
Addiction biology, 26(3), e12947-e12947 (2020-08-05)
Substance use disorder is a complex disease created in part by maladaptive learning and memory mechanisms following repeated drug use. Exposure to drug-associated stimuli engages prefrontal cortex circuits, and dysfunction of the medial prefrontal cortex (mPFC) is thought to underlie

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