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Merck

SAB4200354

Sigma-Aldrich

Anti-SMAD2 antibody produced in rabbit

~1.0 mg/mL, affinity isolated antibody

Sinónimos:

Anti-JV18, Anti-JV18-1, Anti-MADH2, Anti-MADR2, Anti-Mothers against decapentaplegic homolog 2, Anti-hMAD-2, Anti-hSMAD2

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About This Item

Código UNSPSC:
12352203
NACRES:
NA.41

origen biológico

rabbit

conjugado

unconjugated

forma del anticuerpo

affinity isolated antibody

tipo de anticuerpo

primary antibodies

clon

polyclonal

formulario

buffered aqueous solution

mol peso

antigen ~52 kDa

reactividad de especies

human

concentración

~1.0 mg/mL

técnicas

indirect immunofluorescence: 2.5-5.0 μg/mL using using human HeLa cells
western blot: 2-4 μg/mL using whole extracts of HEK-293T cells over-expressing human SMAD2

Nº de acceso UniProt

Condiciones de envío

dry ice

temp. de almacenamiento

−20°C

modificación del objetivo postraduccional

unmodified

Información sobre el gen

human ... SMAD2(4087)
mouse ... Smad2(17126)
rat ... Smad2(29357)

Descripción general

SMAD2 belongs to the SMAD family of proteins. The members of this family contain two conserved domains, the N-terminal MH1 and C-terminal MH2, that are separated by a proline-rich linker region of varying length. The MH1 domain is implicated in the regulation of nuclear import and transcription by binding to DNA and interacting with nuclear proteins. The MH2 domain is involved in the modulation of Smad oligomerization and recognition by type I receptors and it associated with cytoplasmic adaptors and transcription factors.
The SMAD2 gene is located on the human chromosome at 18q21.1.

Especificidad

Anti-SMAD2 recognizes human SMAD2.

Inmunógeno

peptide corresponding to an internal region of human SMAD2, conjugated to KLH. The corresponding sequence is identical in mouse, rat and bovine.

Aplicación

Anti-SMAD2 antibody produced in rabbit may be used in immunoblotting and immunofluorescence.

Acciones bioquímicas o fisiológicas

Members of the SMAD family are essential for transmitting signals from the transforming growth factor-β (TGFβ) to the nucleus, and thus regulate various cellular processes, including cell proliferation, apoptosis, and differentiation. Smad4 is essential for modulating the transactivation efficacy of the Smad complexes in the nucleus. SMAD2 is recruited to TGFβ receptors through its interaction with the SARA (SMAD anchor for receptor activation) protein. In response to TGFβ, SMAD2 is phosphorylated by TGFβ receptors. This phosphorylation induces the dissociation of SMAD2 from SARA and the association with the family member SMAD4.The association with SMAD4 is important for the translocation of SMAD2 into the nucleus, where it binds to target promoters and forms a transcription repressor complex with other cofactors.

Forma física

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Almacenamiento y estabilidad

For continuous use, store at 2-8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing, or storage in “frost-free” freezers,is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Opcional

Referencia del producto
Descripción
Precios

Código de clase de almacenamiento

10 - Combustible liquids

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable


Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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Smad regulation in TGF-beta signal transduction
Moustakas A, et al.
Journal of Cell Science, 114, 24-24 (2001)
SARA, a FYVE Domain Protein that Recruits Smad2 to the TGFbeta Receptor
Tsukazaki T, et al.
Cell, 95, 779-791 (1998)
Lingfang Zeng et al.
Arteriosclerosis, thrombosis, and vascular biology, 35(10), 2134-2144 (2015-09-01)
Smooth muscle cell (SMC) migration and proliferation play an essential role in neointimal formation after vascular injury. In this study, we intended to investigate whether the X-box-binding protein 1 (XBP1) was involved in these processes. In vivo studies on femoral
Yi Yang et al.
Oncology research, 21(6), 345-352 (2013-01-01)
TGF-β/Smad signaling induces epithelial-mesenchymal transition (EMT) and tumor metastasis. As essential mediators in this pathway, Smad2 and Smad3 have been extensively studied and found to promote EMT and the subsequent mobility as well as invasiveness of lung cancer cells. In
Hye Sook Min et al.
Laboratory investigation; a journal of technical methods and pathology, 94(6), 598-607 (2014-04-02)
Dipeptidyl peptidase IV (DPPIV) is an exopeptidase that modulates the function of several substrates, among which insulin-releasing incretin hormones are the most well known. DPPIV also modulate substrates involved in inflammation, cell migration, and cell differentiation. Although DPPIV is highly

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