SAB4200287

Anti-Actin, Muscle specific antibody, Mouse monoclonal

clone HHF-35, purified from hybridoma cell culture

• Código UNSPSC: 12352203
• NACRES: NA.41

Propiedades

• origen biológico: mouse
• Nivel de calidad: 200
• conjugado: unconjugated
• forma del anticuerpo: purified from hybridoma cell culture
• tipo de anticuerpo: primary antibodies
• clon: HHF-35, monoclonal
• formulario: buffered aqueous solution
• mol peso: antigen ~42 kDa
• reactividad de especies: rat, bovine, canine, chicken, human, mouse, hamster, monkey
• concentración: ~1.0 mg/mL
• técnicas: immunocytochemistry: 2-4 μg/mL using A10 cells; immunohistochemistry: suitable; western blot: 0.1-0.2 μg/mL using A10 cell extracts
• isotipo: IgG1
• Condiciones de envío: dry ice
• temp. de almacenamiento: −20°C
• modificación del objetivo postraduccional: unmodified
• Información sobre el gen: human ... ACTB(60)

Descripción

Descripción general

Monoclonal Anti-Actin, muscle specific (mouse IgG1 isotype) is derived from the hybridoma HHF-35 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with SDS extracted protein fraction of human myocardium. The two major cytoskeletal proteins implicated in cell motility are actin and myosin. Although actin is one of the most conserved eukaryotic proteins, it is expressed in mammals and birds as six isoforms characterized by electrophoretic mobility and amino acid sequence analysis.

Inmunógeno

SDS-extracted protein fraction of human myocardium.

Aplicación

The antibody may be used in various immunochemical techniques including, immunoblotting, immunocytochemistry and immunohistochemistry.

Acciones bioquímicas o fisiológicas

Actin and myosin are constituents of many cells types and are involved in a myriad of cellular processes including locomotion, secretion, cytoplasmic streaming, phagocytosis, and cytokinesis.

Forma física

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Información de seguridad

• Código de clase de almacenamiento: 10 - Combustible liquids
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable