SAB4200036

Anti-ITCH antibody produced in rabbit

~1.5 mg/mL, affinity isolated antibody, buffered aqueous solution

• Sinónimos: Anti-AIP4, Anti-Atrophin-1-interacting protein 4, Anti-Itchy E3 ubiquitin protein ligase homolog, Anti-NAPP1, Anti-NFE2-associated polypeptide 1
• Código UNSPSC: 12352203
• NACRES: NA.41

Propiedades

• origen biológico: rabbit
• Nivel de calidad: 200
• conjugado: unconjugated
• forma del anticuerpo: affinity isolated antibody
• tipo de anticuerpo: primary antibodies
• clon: polyclonal
• formulario: buffered aqueous solution
• mol peso: antigen ~112 kDa
• reactividad de especies: monkey, human
• concentración: ~1.5 mg/mL
• técnicas: western blot: 1.5-3.0 μg/mL using Jurkat and COS7 cell extracts
• Nº de acceso UniProt: Q96J02
• Condiciones de envío: dry ice
• temp. de almacenamiento: −20°C
• modificación del objetivo postraduccional: unmodified
• Información sobre el gen: human ... ITCH(83737)

Descripción

Descripción general

Itchy E3 ubiquitin protein ligase (ITCH), also known as atrophin 1-interacting protein 4, is encoded by the gene mapped to human 20q11.22. The encoded protein is a member of the Nedd4-like family of E3 ubiquitin ligases. ITCH is characterized by an N- terminal protein kinase C-related C2 domain, four WW domains and a HECT (homologous to the E6-associated protein carboxyl-terminus) ubiquitin protein ligase domain at the C-terminus.

Especificidad

Anti-ITCH specifically recognizes human and monkey ITCH.

Aplicación

Anti-ITCH antibody produced in rabbit has been used in western blotting and immunoprecipitation.

Acciones bioquímicas o fisiológicas

ITCH can act as a transcriptional corepressor of nuclear factor erythroid 2 (NFE2)/p45. This protein may participate in mediating the immune responses by modifying Notch-mediated signaling.

Itchy E3 ubiquitin protein ligase (ITCH) in association with Ebola virus VP40 (eVP40), aids in virus-like particles (VLP) and virus budding. Overexpression of the gene is associated with the development of thyroid tumors, mainly anaplastic thyroid carcinoma (ATC). ITCH inhibits hippo tumor suppressor pathway and induce metastasis in breast cancer patients. The encoded protein is implicated in the positive regulation of transforming growth factor-β (TGF-β) signaling and of following epithelial-mesenchymal transition (EMT)-related gene expression. Down-regulated expression of the gene leads to syndromic multisystem autoimmune disease and severe morphologic and developmental abnormalities.

Forma física

Solution in 0.01 M phos­phate buffered saline, pH 7.4, containing 15 mM sodium azide.

Almacenamiento y estabilidad

Store at –20 °C. For continuous use, the product may be stored at 2–8 °C for up to one month. For extended storage, freeze in working aliquots at –20 °C. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Información de seguridad

• Código de clase de almacenamiento: 10 - Combustible liquids
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable