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SAB2702218

Sigma-Aldrich

Monoclonal Anti-6X His tag antibody produced in mouse

clone GT359, affinity isolated antibody

Sinónimos:

Mouse Anti-Hexa Histidine tag

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About This Item

Código UNSPSC:
12352203
NACRES:
NA.43

origen biológico

mouse

Nivel de calidad

conjugado

unconjugated

forma del anticuerpo

affinity isolated antibody

tipo de anticuerpo

primary antibodies

clon

GT359, monoclonal

Formulario

buffered aqueous solution

reactividad de especies

human, mouse

concentración

1mg/mL

técnicas

ELISA: suitable
immunofluorescence: suitable
immunoprecipitation (IP): suitable
western blot: 500-3000

isotipo

IgG3

Condiciones de envío

wet ice

temp. de almacenamiento

−20°C

modificación del objetivo postraduccional

unmodified

Descripción general

6X His is a histidine tag that consists of a series of histidine residues at the amino or carboxy terminus of a protein.

Inmunógeno

The immunogen used to generate this antibody corresponds to 6X His tag

Aplicación

Suggested starting dilutions are as follows: ELISA: 1:1000-1:10000, ICC/IF: 1:100-1:2000, IP: 1:100-1:500, WB: 1:500-1:3000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.

Monoclonal Anti-6X His tag antibody produced in mouse has been used in Co-Immunoprecipitation (Co-IP) and immunoblotting.

Acciones bioquímicas o fisiológicas

6X His tag does not affect the structure and function of protein and can be used in antibody production. It is also used to purify proteins under native or denaturing form.

Características y beneficios

Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more.

Otras notas

Purification: Affinity purified by Protein G

Forma física

Phosphate-buffered saline, no preservative added.

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Código de clase de almacenamiento

10 - Combustible liquids

Clase de riesgo para el agua (WGK)

WGK 1

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable


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Disrupting CD147-RAP2 interaction abrogates erythrocyte invasion by Plasmodium falciparum.
Z Meng-Yao, et al.
Blood, 131(10), 1111-1121 (2018)
SKP2-and OTUD1-regulated non-proteolytic ubiquitination of YAP promotes YAP nuclear localization and activity.
Yao F, et al.
Nature Communications, 9(1), 2269-2269 (2018)
Jordan Demone et al.
PloS one, 17(12), e0277668-e0277668 (2022-12-15)
Agroinfiltration is a method used in biopharming to support plant-based biosynthesis of therapeutic proteins such as antibodies and viral antigens involved in vaccines. Major advantages of generating proteins in plants is the low cost, massive scalability and the rapid yield
Molecular Biology of Streptococci (2007)
Meng-Yao Zhang et al.
Blood, 131(10), 1111-1121 (2018-01-21)
Effective vaccines against malaria caused by Plasmodium falciparum are still lacking, and the molecular mechanism of the host-parasite interaction is not fully understood. Here we demonstrate that the interaction of RAP2, a parasite-secreted rhoptry protein that functions in the parasitophorous

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