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SAB2702215

Sigma-Aldrich

Monoclonal Anti-Strep Tag antibody produced in mouse

clone GT517, affinity isolated antibody

Sinónimos:

Mouse Anti-Strep Tag, Strep Tag Detection Antibody

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About This Item

Código UNSPSC:
12352203
NACRES:
NA.43
conjugado:
unconjugated
application:
IF
WB
clon:
GT517, monoclonal
técnicas:
indirect immunofluorescence: suitable
western blot: 1000-10000
citations:
8

origen biológico

mouse

Nivel de calidad

conjugado

unconjugated

forma del anticuerpo

affinity isolated antibody

tipo de anticuerpo

primary antibodies

clon

GT517, monoclonal

Formulario

buffered aqueous solution

concentración

1mg/mL

técnicas

indirect immunofluorescence: suitable
western blot: 1000-10000

isotipo

IgG1

Condiciones de envío

wet ice

temp. de almacenamiento

−20°C

modificación del objetivo postraduccional

unmodified

Descripción general

The strep-tag/strep-tagII (improved version) is made up of eight amino acid sequence (Trp-Ser-His-Pro-Gln-Phe-Glu-Lys).

Inmunógeno

The immunogen used to generate this antibody corresponds to Strep Tag

Aplicación

Suggested starting dilutions are as follows: ICC/IF: 1:100-1:1000, WB: 1:1000-1:10000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.

Acciones bioquímicas o fisiológicas

The strep-tag/strep-tagII (improved version) has the ability to bind to any protein or peptide of interest.

Características y beneficios

Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more.

Otras notas

Purification: Affinity purified by Protein G

Forma física

Phosphate-buffered saline, no preservative added.

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Código de clase de almacenamiento

12 - Non Combustible Liquids

Clase de riesgo para el agua (WGK)

nwg

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable


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The Strep-tag system for one-step purification and high-affinity detection or capturing of proteins.
Schmidt TG and Skerra A
Nature Protocols, 2(6), 1528-1528 (2007)
Zhaofeng Yan et al.
Nature communications, 11(1), 241-241 (2020-01-15)
A major component of bacterial biofilms is curli amyloid fibrils secreted by the curli biogenesis system. Understanding the curli biogenesis mechanism is critical for developing therapeutic agents for biofilm-related infections. Here we report a systematic study of the curli biogenesis
The Strep-tag system for one-step affinity purification of proteins from mammalian cell culture.
Schmidt T and Skerra A
Methods in Molecular Biology, 1286, 83-95 (2015)
Timothy A Bates et al.
Cell reports, 34(7), 108737-108737 (2021-02-06)
In the ongoing coronavirus disease 2019 (COVID-19) pandemic, there remain unanswered questions regarding the nature and significance of the humoral immune response toward other coronavirus infections. Here, we investigate the cross-reactivity of antibodies raised against the first severe acute respiratory
Yongqiang Lai et al.
Scientific reports, 9(1), 14257-14257 (2019-10-05)
Replication protein A (RPA) binds to and stabilizes single-stranded DNA and is essential for the genome stability. We reported that an E3 ubiquitin ligase, HERC2, suppresses G-quadruplex (G4) DNA by regulating RPA-helicase complexes. However, the precise mechanism of HERC2 on

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