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S6147

Sigma-Aldrich

Sephadex® G-100

BioReagent, for molecular biology, DNA grade, medium

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About This Item

Número de CAS:
9050-94-6
Número MDL:
MFCD00132235
Código UNSPSC:
23151817
NACRES:
NA.52

grado

DNA grade
for molecular biology

Línea del producto

BioReagent

formulario

beads

técnicas

buffer exchange: suitable

grupo activo de la matriz

phase

hinchazón

1 g swells to 9-11 mL

diámetro microesfera

40-120 μm

tamaño de poro

exclusion limit (25 bp dsDNA; (rA)27)

pH

2-10

aplicaciones

life science and biopharma

compatibilidad

Cytiva

actividad extraña

DNase, RNase, none detected

temp. de almacenamiento

room temp

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Descripción general

Sephadex® G-100, Medium is an often-cited gel filtration medium for desalting and buffer exchange of very large molecules. Sephadex® is prepared by crosslinking dextran with epichlorohydrin. Sephadex products differ in their degree of cross-linking and hence in their degree of swelling and their molecular fractionation range. “Medium” indicates a large particle size for use in large-scale group separations where high flow rates and low operating pressures are required.

Aplicación

Sephadex® G-100 is suitable for spin column purification of DNA (from contaminating small molecules) for use in hybridizations. It was used to purify the enzyme polyphenoloxidases isolated from banana by gel filteration.

Características y beneficios

  • Excellent recovery
  • Minimal sample dilution
  • Quick and easy to use

Información legal

Sephadex is a registered trademark of Cytiva

Producto relacionado

Referencia del producto
Descripción
Precios

Código de clase de almacenamiento

11 - Combustible Solids

Clase de riesgo para el agua (WGK)

WGK 3

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable

Equipo de protección personal

Eyeshields, Gloves, type N95 (US)

Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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Isolation, purification and physicochemical characterization of polyphenoloxidases (PPO) from a dwarf variety of banana (Musa cavendishii, L)
Galeazzi MAM et al
Journal of Food Science, 46, 150-155 (1981)
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