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Merck

S5447

Sigma-Aldrich

Anti-SIRT1 antibody produced in rabbit

~1.0 mg/mL, affinity isolated antibody, buffered aqueous solution

Sinónimos:

Anti-S. cerevisiae, HOMOLOG-LIKE 1, Anti-SIR2, Anti-SIR2-α, Anti-SIR2L1, Anti-Sirtuin 1

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About This Item

Número MDL:
Código UNSPSC:
12352203
NACRES:
NA.41

origen biológico

rabbit

conjugado

unconjugated

forma del anticuerpo

affinity isolated antibody

tipo de anticuerpo

primary antibodies

clon

polyclonal

formulario

buffered aqueous solution

mol peso

antigen ~120 kDa

reactividad de especies

human

concentración

~1.0 mg/mL

técnicas

immunoprecipitation (IP): 5-10 μg using extracts of Jurkat cells
western blot: 2.5-5 μg/mL using whole extracts of Jurkat cells

Nº de acceso UniProt

Condiciones de envío

dry ice

temp. de almacenamiento

−20°C

modificación del objetivo postraduccional

unmodified

Información sobre el gen

human ... SIRT1(23411)
mouse ... Sirt1(93759)
rat ... Sirt1(309757)

Descripción general

Anti-SIRT1 recognizes human SIRT1. Sirtuin 1 (SIRT1) is encoded by the gene mapped to human chromosome 10q21.3. The encoded protein belongs to the silent information regulator (SIR) family and is a class of HDAC (histone deacetylase). SIRT1 is expressed in various tissues including brain, liver, pancreas, adipose tissue, and skeletal muscle. SIRT1 is also known as the longevity gene.

Inmunógeno

synthetic peptide corresponding to amino acids 580-593 of human SIRT1, conjugated to KLH via an N-terminal cysteine residue. The corresponding sequence differs by three amino acids in rat and mouse Sirt1.

Aplicación

Anti-SIRT1 antibody produced in rabbit has been used in immunohistochemistry.

Acciones bioquímicas o fisiológicas

Sirtuin 1 (SIRT1) functions as a regulator of various metabolic pathways, and influences the pathophysiology of several metabolic diseases. It is a regulator of protein deacetylation, and is a candidate therapeutic target in non-alcoholic fatty liver disease (NAFLD), amyotrophic lateral sclerosis (ALS), kidney disease, and pulmonary disease. It also participates in tumorigenesis, and whether it functions as an oncogene or as a tumor suppressor depends upon the tumor type. In pancreatic ductal adenocarcinoma (PDAC), its elevated expression is linked with poor prognosis, and in non-small-cell lung cancer (NSCLC), it suppresses the expression of tumor suppressor p27. The encoded protein inhibits the terminal differentiation in dividing myocytes by binding to the myoD cofactor, pCAF. It is also thought to function as a suppressor of cardiovascular disorders, such as myocardial infarction, or neurodegenerative diseases, such as Alzheimer′s disease (AD) or Parkinson′s disorder (PD).

Forma física

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Almacenamiento y estabilidad

For continuous use, store at 2-8°C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing, or storage in “frostfree” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.

Otras notas

In order to obtain the best results using various techniques and preparations, we recommend determining the optimal working dilutions by titration.

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Código de clase de almacenamiento

10 - Combustible liquids

Clase de riesgo para el agua (WGK)

WGK 3

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable

Equipo de protección personal

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)


Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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Visite la Librería de documentos

Cloning, chromosomal characterization and mapping of the NAD-dependent histone deacetylases gene sirtuin 1.
Voelter-Mahlknecht S and Mahlknecht U
International Journal of Molecular Medicine, 17, 59-67 (2006)
17?-Estradiol Attenuates Hematoma Expansion through ERa/Sirt1/NF-?B Pathway in Hyperglycemic Intracerebral Hemorrhage Mice
Zheng Y, et al.
Stroke, 46, 485?491-485?491 (2015)
Mammalian SIRT1 Represses Forkhead Transcription Factors
Motta MC, et al.
Cell, 116, 551-563 (2004)
SIRT 1 Overexpression is Associated with Metastasis of Pancreatic Ductal Adenocarcinoma (PDAC) and Promotes Migration and Growth of PDAC Cells.
Li S
Medical Science Monitor : International Medical Journal of Experimental and Clinical Research, 22 (2016)
[SIRT1].
Hattori Y and Ihara M
Nihon Rinsho. Japanese Journal of Clinical Medicine (2016)

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