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S500HR

Sigma-Aldrich

Sephacryl® Size Exclusion Resin

500-HR, MW range 40-20,000 kDa (dextrans)

Sinónimos:

Poly([allyl dextran]-co-N,N′-methylenebisacrylamide)

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About This Item

Número de CAS:
65546-95-4
Número MDL:
MFCD00165824
Código UNSPSC:
23151817
NACRES:
NA.56

Nombre del producto

Sephacryl®, 500-HR, MW range 40-20,000 kDa (dextrans)

Nivel de calidad

100

descripción

stability: stable to 0.2M NaOH

Formulario

suspension (20% aqueous ethanol)

Características

autoclavable

Matriz

crosslinked allyl dextran/N,N′-methylenebisacrylamide copolymer

tamaño de partícula

25-75 μm (wet)

tamaño de poro

40-20,000 kDa MW range (dextrans)

compatibilidad

mode of use size exclusion chromatography

temp. de almacenamiento

2-8°C

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Descripción general

  • Supplied swollen, suspended in 20% aqueous ethanol
  • Autoclavable
  • Stable to 0.2N sodium hydroxide (NaOH)
  • Store at refrigerated temperature (2-8 °C)
S500HR-750ML′s updated product number is GE17-0613-01

Aplicación

Sephacryl® has been used in size exclusion chromatography purification of the Acacia tortuosa gum, dextran-RBA12 from Weissella cibaria and dextran from Weissella cibaria JAG8.
Sephacryl® is used in gel filtration chromatography, protein chromatography, and resins and separation media. Sephacryl® has been used to develop a novel high-throughput screening assay for sickle cell disease drug discovery. Sephacryl® has also been used to purify and characterize ovine pancreatic elastase as well as a lysozyme from human urine.
For size exclusion chromatography; the HR grade is a smaller particle size with narrower size range distribution, allowing for more efficient separations at faster flow rates. Recommended fractionation ranges are listed for globular proteins and dextrans..

Información legal

Sephacryl is a registered trademark of Cytiva

Pictogramas

Flame
GHS02

Palabra de señalización

Warning

Frases de peligro

H226

Clasificaciones de peligro

Flam. Liq. 3

Código de clase de almacenamiento

3 - Flammable liquids

Clase de riesgo para el agua (WGK)

WGK 1

Punto de inflamabilidad (°F)

100.4 - 109.4 °F - closed cup

Punto de inflamabilidad (°C)

38 - 43 °C - closed cup

Equipo de protección personal

Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter

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Certificados de análisis (COA)

Lot/Batch Number
MKBS9866V
MKBM8415V
SLBB6826V
109K1525
068K1391

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Si necesita una versión concreta, puede buscar un certificado específico por el número de lote.

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M A Karymov et al.
FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 15(14), 2631-2641 (2001-12-01)
Dynamic alterations in chromatin structure mediated by postsynthetic histone modifications and DNA methylation constitute a major regulatory mechanism in DNA functioning. DNA methylation has been implicated in transcriptional silencing, in part by inducing chromatin condensation. To understand the methylation-dependent chromatin
Theresa L Gioannini et al.
The Journal of biological chemistry, 277(49), 47818-47825 (2002-10-10)
Experiments utilizing endotoxin aggregates, lipooligosaccharides (LOS) isolated from metabolically labeled Neisseria meningitidis serotype group B, demonstrate that albumin is an essential component of lipopolysaccharide binding protein- (LBP) and sCD14-dependent 1) disaggregation of LOS and 2) LOS activation of human umbilical
Structural and biocompatibility properties of dextran from Weissella cibaria JAG8 as food additive
Tingirikari JM, et al.
International Journal of Food Sciences and Nutrition, 65(6), 686-691 (2014)
Xiaolei Wang et al.
Molecular microbiology, 83(1), 195-207 (2011-11-25)
Stewartan and amylovoran exopolysaccharide (EPS) produced by the plant pathogenic bacteria Pantoea stewartii and Erwinia amylovora are virulence factors in the cause of Stewart's vascular wilt and fire blight. The biosynthesis of amylovoran and stewartan is encoded by a set
Takashi Ideue et al.
Genes & development, 21(16), 1993-1998 (2007-08-07)
Pre-mRNA splicing specifically deposits the exon junction complex (EJC) onto spliced mRNA, which is important for downstream events. Here, we show that EJC components are primarily recruited to the spliceosome by association with the intron via the intron-binding protein, IBP160.
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