S4945

Anti-SynCAM antibody produced in rabbit

affinity isolated antibody, buffered aqueous solution

• Sinónimos: Anti-IGSF4, Anti-Necl-2, Anti-RAI75, Anti-SgIGSF, Anti-Tslc1
• Número MDL: MFCD07370522
• Código UNSPSC: 12352203
• NACRES: NA.41

Propiedades

• origen biológico: rabbit
• Nivel de calidad: 200
• conjugado: unconjugated
• forma del anticuerpo: affinity isolated antibody
• tipo de anticuerpo: primary antibodies
• clon: polyclonal
• Formulario: buffered aqueous solution
• mol peso: antigen 48 kDa
• reactividad de especies: mouse, rat
• técnicas: immunohistochemistry: suitable using suitable for IHC on rat brain perfused with 4% paraformaldehyde; microarray: suitable; western blot: 0.5-1.0 μg/mL using mouse brain and rat brain extract (S1 fraction)
• Nº de acceso UniProt: Q8R5M8
• Condiciones de envío: dry ice
• temp. de almacenamiento: −20°C
• modificación del objetivo postraduccional: unmodified
• Información sobre el gen: mouse ... Cadm1(54725) ; rat ... Cadm1(363058)

Descripción

Descripción general

Anti-SynCAM is developed in rabbit using a synthetic peptide corresponding to amino acids located at the C-terminus of mouse SynCAM1 conjugated to keyhole limpet hemocyanin (KLH) as immunogen. Synaptic cell adhesion molecule (SynCAM) is a brain-specific protein which has an immunoglobulin domain. It binds to intracellular PDZ-domain protein. SynCAMs have also been called nectin-like molecules (Necls). SynCAM1 is also known as Necl-2/ IGSF4/ RA175/ SgIGSF/ TSLC1. SynCAM contains an N-terminal signal peptide, three extracellular Ig domains, a single transmembrane region and a short C-terminal cytoplasmic domain. It is expressed only in brain and is localized to synapses.

SynCAM proteins consist of a C-terminal cytoplasmic domain, an N-terminal signaling domain and three extracellular immunoglobulin domains.. Anti-SynCAM antibody is specific for SynCAM in mouse and rats. The SynCAM1 immunizing peptide (mouse, amino acids 431-445) specifically inhibits the staining of the SynCAM band in immunoblotting.

Inmunógeno

This immunogen sequence is identical in human SynCAM1, highly conserved (90%) in the splice variant SynCAM2 and displays limited homology (55%) to SynCAM3.

synthetic peptide corresponding to amino acids 431-445 located at the C-terminus of mouse SynCAM1 conjugated to KLH.

Aplicación

• Anti-SynCAM antibody produced in rabbit has been used in:
• immunoprecipitation
• immunoblotting
• immunohistochemistry
• immunostaining
• immunofluorescence

Acciones bioquímicas o fisiológicas

SynCAM is a neurological cell-adhesion protein that regulates the formation of synapses. Expression of synaptic cell adhesion molecule (SynCAM) in non-neuronal cells induces synaptic formation and affects synaptic function in hippocampal neurons. It functions as a homophilic cell adhesion molecule at the synapse. SynCAM is believed to interact with PDZ-domain synaptic proteins CASK and syntenin via its cytoplasmic tail.

Forma física

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Información de seguridad

• Código de clase de almacenamiento: 10 - Combustible liquids
• Clase de riesgo para el agua (WGK): WGK 3
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable
• Equipo de protección personal: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)