S4014

Spectinomycin dihydrochloride pentahydrate

powder, suitable for cell culture and plant cell culture, BioReagent

• Sinónimos: Spectinomycin
• Fórmula empírica (notación de Hill): C₁₄H₂₄N₂O₇ · 2HCl · 5H₂O
• Número de CAS: 22189-32-8
• Peso molecular: 495.35
• Número CE: 244-554-3
• Número MDL: MFCD00150886
• Código UNSPSC: 12352207
• ID de la sustancia en PubChem: 24899581
• NACRES: NA.76

Propiedades

• product name: Spectinomycin dihydrochloride pentahydrate, powder, BioReagent, suitable for cell culture
• origen biológico: synthetic (inorganic)
• Nivel de calidad: 200
• Línea del producto: BioReagent
• formulario: powder
• potencia: ≥603 μg per mg
• condiciones de almacenamiento: (Keep container tightly closed in a dry and well-ventilated place. Keep in a dry place.)
• técnicas: cell culture | mammalian: suitable; cell culture | plant: suitable
• impurezas: endotoxin, tested
• color: white to pale buff
• solubilidad: H₂O: soluble 50 mg/mL at
• espectro de actividad antibiótica: Gram-negative bacteria; Gram-positive bacteria
• aplicaciones: agriculture
• Modo de acción: protein synthesis | interferes
• temp. de almacenamiento: 2-8°C
• cadena SMILES: O.O.O.O.O.Cl.Cl.CN[C@@H]1[C@H](O)[C@H](NC)[C@H]2O[C@]3(O)[C@@H](O[C@H](C)CC3=O)O[C@@H]2[C@H]1O
• InChI: 1S/C14H24N2O7.2ClH.5H2O/c1-5-4-6(17)14(20)13(21-5)22-12-10(19)7(15-2)9(18)8(16-3)11(12)23-14;;;;;;;/h5,7-13,15-16,18-20H,4H2,1-3H3;2*1H;5*1H2/t5-,7-,8+,9+,10+,11-,12-,13+,14+;;;;;;;/m1......./s1
• Clave InChI: DCHJOVNPPSBWHK-UXXUFHFZSA-N

Descripción

Descripción general

Chemical structure: aminoglycoside

Spectinomycin has generally been used for the treatment of gonorrhea in males, and a study conducted shows that spectinomycin dihydrochloride pentahydrate provides effective treatment against gonorrhea as well. Clinically, spectinomycin dihydrochloride pentahydrate is easier to use in injections as it shows higher solubility than spectinomycin.

Aplicación

Spectinomycin dihydrochloride pentahydrate has been used as a supplement in different types of media.

Spectinomycin is an aminocyclitol antibiotic derived from Streptomyces spectabilis. It has been shown to have a wide variety of uses, including treating acute gonorrheal urethritis and cervictis, to mark cell layers to monitor cell fate during leaf development, as a selection marker in plant related transformation systems for plant cells containing the marker gene Spcr, to study respiratory tract infections of cattle caused by Pasteurella multocida and Mannheimia haemolytica, and to generate plants deficient for the plastid-encoded RNA polymerase on MS-agar media. Spectinomycin also has use in amplification of low copy number plasmid carrying replicons. It is recommended for use in cell culture applications at 7.5-20 mg/L.

Acciones bioquímicas o fisiológicas

Mode of Action: Spectinomycin acts by inhibiting protein synthesis and elongation by binding to the bacterial 30S ribosomal subunit and interfering with peptidyl tRNA translocation.

Mode of Resistance: A mutation in rpsE, the gene for ribosomal protein S5, prevents the binding of spectinomycin. Resistance is also conferred by the aminoglycoside-3′-adenyltransferase gene (aadA).

Antimicrobial spectrum: Spectinomycin acts against gram-negative and gram-positive bacteria.

Precaución

Solutions can be stored at 2-8°C for several weeks or at -20°C for more extended periods.

Nota de preparación

Stock solutions should be prepared at 10 mg/mL in water and filter sterilized.

Otras notas

Keep container tightly closed in a dry and well-ventilated place. Keep in a dry place.

Información de seguridad

• Pictogramas: GHS07
• Palabra de señalización: Warning
• Frases de peligro: H315,H319,H335
• Consejos de prudencia: P261 - P264 - P271 - P280 - P302 + P352 - P305 + P351 + P338
• Clasificaciones de peligro: Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3
• Órganos de actuación: Respiratory system
• Código de clase de almacenamiento: 11 - Combustible Solids
• Clase de riesgo para el agua (WGK): WGK 2
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable
• Equipo de protección personal: Eyeshields, Gloves, type N95 (US)