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RAB0081

Sigma-Aldrich

Human CD14 ELISA Kit

for serum, plasma, cell culture supernatant and urine

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About This Item

Código UNSPSC:
41116158
NACRES:
NA.32

reactividad de especies

human

envase

kit of 96 wells (12 strips x 8 wells)

técnicas

ELISA: suitable
capture ELISA: suitable

entrada

sample type cell culture supernatant(s)
sample type urine
sample type plasma
sample type serum

assay range

inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 6 pg/mL
standard curve range: 8.23-6000 pg/mL

método de detección

colorimetric

Condiciones de envío

wet ice

temp. de almacenamiento

−20°C

Información sobre el gen

human ... CD14(929)

Descripción general

The Human CD14 ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human CD14 in serum, plasma, cell culture supernatants and urine.

Inmunógeno

Recombinant Human CD14

Aplicación

For research use only. Not for use in diagnostic procedures.
Please refer to the attached General ELISA KIT Procedure (sandwich, competitive & Indirect ELISA)

Acciones bioquímicas o fisiológicas

Cluster of differentiation 14 (CD14) is present on the dendritic cells (DCs), macrophages, and monocytes. It is a glycoprotein that lacks a transmembrane domain and is attached to the cell surface by a glycosylphosphatidylinositol (GPI) anchor. CD14 plays a crucial role in the recognition of bacteria and in immune reactivation. It also plays a key role in host responses related to the recognition of viral components by the lungs. The Human CD14 enzyme-linked immunosorbent assay (ELISA) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human CD14 in serum, plasma, cell culture supernatants, and urine.

Otras notas

A sample Certificate of Analysis is available for this product.
Please type the word sample in the text box provided for lot number.

Los componentes del kit también están disponibles por separado

Referencia del producto
Descripción
SDS

  • RABELADEELISA 5X Assay/Sample Diluent Buffer E (Item E2)SDS

  • RABSTOP3ELISA Stop Solution (Item I)SDS

  • RABTMB3ELISA Colorimetric TMB Reagent (HRP Substrate, Item H)SDS

  • RABWASH420X Wash Buffer (Item B)SDS

Pictogramas

Corrosion

Palabra de señalización

Warning

Frases de peligro

Consejos de prudencia

Clasificaciones de peligro

Met. Corr. 1

Código de clase de almacenamiento

8A - Combustible corrosive hazardous materials


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Thomas Damgaard Sandahl et al.
The American journal of gastroenterology, 109(11), 1749-1756 (2014-08-27)
Inflammatory activation of resident hepatic macrophages (Kupffer cells) by portal-derived lipopolysaccharide (LPS) has a primary role in animal models of alcoholic liver disease, but it has not been systematically or longitudinally studied in human alcoholic hepatitis (AH). We followed 50
Fatemeh Zamani et al.
Advanced pharmaceutical bulletin, 3(2), 329-332 (2013-12-07)
CD14, one of the main differentiation markers on the surface of myeloid lineage cells, acts as a key role in activation of LPS-induced monocytes. LPS (lipopolysaccharide) binds to LPS-binding protein in plasma and are delivered to the cell surface receptor
Megan Crane et al.
The Journal of infectious diseases, 210(5), 745-751 (2014-03-04)
We investigated the relationship between microbial translocation, immune activation, and liver disease in human immunodeficiency virus (HIV)/hepatitis B virus (HBV) coinfection. Lipopolysaccharide (LPS), soluble CD14, CXCL10, and CCL-2 levels were elevated in patients with HIV/HBV coinfection. Levels of LPS, soluble
Laure Campillo-Gimenez et al.
The Journal of allergy and clinical immunology, 134(5), 1142-1152 (2014-07-22)
Despite control of HIV infection under antiretroviral therapy (ART), immune T-cell activation persists in patients with controlled HIV infection, who are at higher risk of inflammatory diseases than the general population. PMNs play a key role in host defenses against

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