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Merck

R5636

Sigma-Aldrich

Ribonucleic acid, transfer from baker′s yeast (S. cerevisiae)

buffered aqueous solution

Sinónimos:

Transfer RNA, tRNA

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About This Item

Número de CAS:
Número MDL:
Código UNSPSC:
12352106
NACRES:
NA.55

grado

for molecular biology

Nivel de calidad

formulario

buffered aqueous solution

concentración

≥9 mg/mL

actividad extraña

DNase, Nickase, none detected

Condiciones de envío

dry ice

temp. de almacenamiento

−20°C

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Descripción general

Transfer RNA (tRNA) is isolated from baker′s yeast by phenol-chloroform extraction and ethanol precipitation. tRNAs are approximately 80 nucleotides long RNA molecules with a cloverleaf shaped secondary structure and a tertiary L-shaped structure.

Aplicación

Suitable for use as a carrier in nucleic acid purification and precipitation.
Ribonucleic acid, transfer from baker′s yeast (S. cerevisiae) has been used:
  • in carrier solution as a part of control used for reverse transcriptase – quantitative polymerase reaction (RT-qPCR) assay
  • as a component of prehybridization(4) and hybridization buffer in single-label in situ hybridization
  • as a carrier to enhance recovery of RNA from small numbers of cells

Acciones bioquímicas o fisiológicas

Transfer RNAs (tRNAs) play an important role in translation. It is responsible for the addition of amino acids to ribosome for peptide chain formation. The tRNA contains an anticodon region for mRNA base pairing and two attachment regions: for amino acid binding and tRNA synthetase recognition. The ribosomal RNA facilitates the movement of tRNA along the mRNA.

Componentes

tRNA is provided in a solution in 10 mM Tris HCl (pH 7.4) in 1 mM EDTA.

Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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