R354-05A

Rat Aortic Smooth Muscle Cells: RAOSMC, adult

• Código UNSPSC: 41106514
• NACRES: NA.81

Propiedades

• origen biológico: rat aorta (healthy adult tunica intima and media)
• envase: pkg of 500,000 cells
• fabricante / nombre comercial: Cell Applications, Inc
• modo de crecimiento: Adherent
• morfología: smooth muscle
• técnicas: cell culture | mammalian: suitable
• enfermedades relevantes: cardiovascular diseases
• Condiciones de envío: dry ice
• temp. de almacenamiento: −196°C

Descripción

Descripción general

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RAOSMC provide an excellent model system to study all aspects of cardiovascular function and disease, especially those related to mechanisms of hyperplasia and hypertrophy of intimal smooth muscle cells leading to vascular occlusion in atherosclerosis and stent restenosis.

RAOSMC from Cell Applications, Inc. have been utilized in dozens of research studies to:

• Provide a gold standard control for smooth muscle markers Ͱ-SMA, calponin and caldesmon expression (Imamura, 2010)
• Elucidate cytokines and growth factors signaling pathways implicated in the molecular regulation of smooth muscle cell proliferation, migration, and overall vascular function (Alexis, 2009; Liu, 2009; Tan, 2009; Ko, 2012; Malabanan, 2012; Santiago, 2012; Newaz, 2013); and demonstrate that peach extract is able to inhibit Ang II-induced intracellular Ca2+ elevation and the generation of ROS, thus showing promise in prevention of cardiovascular diseases (Kono, 2013)
• Investigate extracellular matrix properties and its role in cardiovascular health, repair of damaged vasculature and successful tissue engineering (Chiang, 2009; Kothapalli, 2009a,b,c)
• Demonstrate that hyperglycemia-induced elastin degradation products lead to vascular smooth muscle cell calcification and identify periostin, also implicated in mechanical stress and inflammation responses, as an early marker for this process (Shanmugam, 2013; Sinha, 2013; Yamashita, 2013)
• Develop advanced stent technology, including novel surface materials, and drug delivery systems to prevent restenosis and other occlusive vasculopathies (Taite, 2011; Midwinter, 2012; Li, 2013)

Characterization: positive for smooth muscle cell specific alpha-actin expression.

Origen línea celular

Aorta

Aplicación

Aortic wall contraction, role of smc under normal conditions, blood pressure, distribution of oxygenated blood through systemic circulation.

Componentes

Rat Smooth Muscle Cell Basal Medium that contains 10% FBS and 10% DMSO

Calidad

Each lot was tested for proper morphology, Population Doublings, Negative for HIV, Hepatitis B, Hepatitis C, mycoplasma, bacteria, and fungi.

Nota de preparación

• 2nd passage, >500,000 cells in Rat Smooth Muscle Cell Basal Medium that contains 10% FBS and 10% DMSO
• Can be cultured at least 16 doublings

Rutina de subcultivo

Please refer to the RAOSMC Culture Protocol.

Información de seguridad

• Código de clase de almacenamiento: 10 - Combustible liquids
• Clase de riesgo para el agua (WGK): WGK 3
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable