R0761
Monoclonal Anti-Rat IgG2a antibody produced in mouse
clone R2A-2, ascites fluid
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About This Item
Productos recomendados
origen biológico
mouse
conjugado
unconjugated
forma del anticuerpo
ascites fluid
tipo de anticuerpo
secondary antibodies
clon
R2A-2, monoclonal
contiene
15 mM sodium azide
técnicas
capture ELISA: suitable
dot blot: suitable
immunocytochemistry: suitable
indirect ELISA: 1:5,000
western blot: suitable
isotipo
IgG1
Condiciones de envío
dry ice
temp. de almacenamiento
−20°C
modificación del objetivo postraduccional
unmodified
Descripción general
Immunoglobulins (IgGs) are glycoprotein antibodies that modulate several immune responses. Rat IgGs have four subclasses, namely, IgG1, IgG2a, IgG2b, and IgG2c.
Monoclonal Anti-Rat IgG2a (mouse IgG1 isotype) is derived from the R2A-2 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with purified rat IgG.
Especificidad
Monoclonal Anti-Rat IgG2a recognizes an epitope located on the heavy chain of rat IgG2a. The antibody detects rat IgG2a derived from normal serum or myeloma proteins, but not the other rat immunoglobulins. Weak cross-reaction is observed with guinea pig immunoglobulins but not with IgG and serum preparations of bovine, cat, chicken, dog, goat, horse, human, mouse, pig, rabbit, or sheep.
Inmunógeno
Purified rat IgG
Aplicación
Monoclonal Anti-Rat IgG2a antibody produced in mouse has been used in:
- enzyme-linked immunosorbent assay (ELISA)
- slot blot immunoassay
- immunoblotting
- dot blot
- immunocytochemistry
Acciones bioquímicas o fisiológicas
Rat immunoglobulins (IgGs) against target proteins are often used as primary antibodies in various research applications. Thus, secondary anti-rat IgG is a useful tool for the analysis of target proteins. Monoclonal Anti-Rat IgG2a antibody is specific for epitope on the heavy chain of rat IgG2a. The antibody localizes the denatured-reduced heavy chain molecule of rat IgG2a (γ2a). The product recognizes rat IgG2a derived from normal serum or myeloma proteins, but does not bind to other rat immunoglobulins. The antibody weakly associates with guinea pig immunoglobulins but does not react with IgG and serum preparations derived from bovine, cat, chicken, dog, goat, horse, human, mouse, pig, rabbit, and sheep.
Forma física
The product is provided as ascites fluid with 0.1% sodium azide as a preservative.
Almacenamiento y estabilidad
For continuous use, store at 0-5 °C. For extended storage freeze in working aliquots. Repeated freezing and thawing is not recommended. Storage in "frost-free" freezers is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use,
Cláusula de descargo de responsabilidad
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Código de clase de almacenamiento
10 - Combustible liquids
Clase de riesgo para el agua (WGK)
nwg
Punto de inflamabilidad (°F)
Not applicable
Punto de inflamabilidad (°C)
Not applicable
Certificados de análisis (COA)
Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»
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