PIC0004

Protease Inhibitor Cocktail

ReadyShield^® ready-to-use solution, for the inhibition of serine, cysteine, acid proteases and aminopeptidases, for use in purification of Histidine-tagged proteins, Non-freezing solution

• Sinónimos: Protease inhibitor cocktail
• Código UNSPSC: 12352200
• NACRES: NA.28

Propiedades

• product name: ReadyShield^® Protease Inhibitor Cocktail, for use in purification of Histidine-tagged proteins, Non-freezing solution
• descripción: Purification of Histidine-Tagged Proteins
• formulario: ready-to-use solution
• calidad: 200
• envase: pkg of 1 mL (in amber polypropylene vial); pkg of 5 mL (in amber polypropylene vial)
• temp. de almacenamiento: −20°C

Descripción

Descripción general

This particular protease inhibitor cocktail omits chelators (e.g., EDTA), to optimize its use for purification of histidine-tagged proteins.1 It contains inhibitors with a broad specificity for serine, cysteine, and acid proteases, and aminopeptidases. The cocktail has been tested on extracts from E. coli and insect cells This cocktail is supplied as a ready-to-use solution using a proprietary non-freezing formulation. ReadyShield PIC0004 is a non-freezing formulation that contains the same inhibitors as is the DMSO based cocktail P8849.

Características y beneficios

• Does not freeze in storage for quick, convenient use.
• Tailored for use with Histidine fusion tagged proteins.
• Suggested as 100 X solution.
• Reclosable screw-top vials.
• Can be stored in cooler for approx. 1 month or room temperature for a week.

Nota de preparación

The recommended dilution of the cocktail in the biological extract is 1 ml of the cocktail per 100 ml of lysate from 20 g (wet weight) of E. coli cells or 10 g (wet weight) of baculovirus-infected insect (Spodoptera frugiperda) cells.

Otras notas

For life science research only. Not for use in diagnostic procedures.

Información legal

ReadyShield is a registered trademark of Merck KGaA, Darmstadt, Germany

Información de seguridad

• Pictogramas: GHS07
• Palabra de señalización: Warning
• Frases de peligro: H315,H319
• Consejos de prudencia: P264 - P280 - P302 + P352 - P305 + P351 + P338 - P332 + P313 - P337 + P313
• Clasificaciones de peligro: Eye Irrit. 2 - Skin Irrit. 2
• Código de clase de almacenamiento: 10 - Combustible liquids
• Clase de riesgo para el agua (WGK): WGK 2
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable