PFM250
GenElute™ Five-Minute Plasmid Miniprep Kit
sufficient for 250 purifications
Sinónimos:
GenElute™ Plasmid Miniprep Kit, Gen Elute
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About This Item
Código UNSPSC:
12352200
NACRES:
NA.52
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uso
sufficient for 250 purifications
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Descripción general
The GenElute Five-Minute Plasmid Miniprep Kit features an ultra-streamlined protocol resulting in high-quality plasmid DNA in about five minutes.
Recombinant E. coli overnight culture in LB (Luria-Bertani) broth is treated briefly (1-2 minutes) with a lysis reagent to effect rapid cell lysis and RNA degradation, without prior removal of culture medium. A binding solution is then mixed with the lysate, and DNA is captured on a silica-based binding column. Impurities are removed by a wash solution and bound plasmid DNA is then eluted in Tris buffer or water, ready for immediate use in various downstream applications. Typical yields of high copy plasmids are 2-6 μg from 400 μL of overnight culture in LB broth.
The GenElute Five-Minute Plasmid Miniprep Kits utilize a novel lysis procedure that eliminates the need to pellet cells from an overnight culture, remove growth media or clear a neutralized lysate. 400 μl of culture is added to a 2 mL collection tube and cells are lysed for 1−2 minutes. A Binding Solution is then added and the entire mixture is passed through a binding column. After washing the column to remove trace contaminants, the plasmid DNA is eluted in 10 mM Tris HCl, pH 8.5. Molecular biology water may be substituted as an elution reagent.
Recombinant E. coli overnight culture in LB (Luria-Bertani) broth is treated briefly (1-2 minutes) with a lysis reagent to effect rapid cell lysis and RNA degradation, without prior removal of culture medium. A binding solution is then mixed with the lysate, and DNA is captured on a silica-based binding column. Impurities are removed by a wash solution and bound plasmid DNA is then eluted in Tris buffer or water, ready for immediate use in various downstream applications. Typical yields of high copy plasmids are 2-6 μg from 400 μL of overnight culture in LB broth.
The GenElute Five-Minute Plasmid Miniprep Kits utilize a novel lysis procedure that eliminates the need to pellet cells from an overnight culture, remove growth media or clear a neutralized lysate. 400 μl of culture is added to a 2 mL collection tube and cells are lysed for 1−2 minutes. A Binding Solution is then added and the entire mixture is passed through a binding column. After washing the column to remove trace contaminants, the plasmid DNA is eluted in 10 mM Tris HCl, pH 8.5. Molecular biology water may be substituted as an elution reagent.
Aplicación
GenElute™ Five-Minute Plasmid Miniprep Kit has been used in the isolation of plasmid.
The GenElute Five-Minute Plasmid Miniprep Kit is intended for rapid preparation of plasmid DNA for use in capillary DNA sequencing, clone screening, restriction digestion, and PCR. For low copy plasmids, the kit can be used to prepare plasmid DNA for use in restriction digestion and PCR.
Características y beneficios
- No pelleting cells or clearing lysates
- Binding column works with any standard laboratory vacuum manifold
- Up to 5 μg of plasmid DNA or enough for 15 sequencing reactions
- DNA suitable for immediate capillary DNA sequencing
Otras notas
For additional information, please see www.sigma-aldrich.com/genelutehp.
Información legal
GenElute is a trademark of Sigma-Aldrich Co. LLC
Producto relacionado
Referencia del producto
Descripción
Precios
Palabra de señalización
Danger
Frases de peligro
Consejos de prudencia
Clasificaciones de peligro
Aquatic Acute 1 - Aquatic Chronic 2 - Eye Dam. 1 - Flam. Liq. 2 - Met. Corr. 1 - Resp. Sens. 1 - Skin Corr. 1C - STOT SE 3
Órganos de actuación
Central nervous system
Riesgos supl.
Código de clase de almacenamiento
3 - Flammable liquids
Punto de inflamabilidad (°F)
69.8 °F
Punto de inflamabilidad (°C)
21 °C
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