P7899
Anti-Peroxidase antibody produced in rabbit
fractionated antiserum, lyophilized powder
Sinónimos:
Rabbit Anti HRP
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About This Item
Productos recomendados
origen biológico
rabbit
Nivel de calidad
conjugado
unconjugated
forma del anticuerpo
fractionated antiserum
tipo de anticuerpo
primary antibodies
clon
polyclonal
Formulario
lyophilized powder
reactividad de especies
horseradish
técnicas
indirect ELISA: 1:100,000-1:200,000
temp. de almacenamiento
2-8°C
modificación del objetivo postraduccional
unmodified
Descripción general
Antiserum is determined to be immunospecific for horseradish peroxidase and does not inhibit the enzymatic activity of the peroxidase.
Peroxidase is a hemoprotein.
The fractionation procedure yields primarily the immunoglobulin fraction of antiserum. If necessary, the fractionated antiserum is adsorbed using solid phase techniques. Antiserum is determined to be immunospecific by immunoelectrophoresis (IEP), against purified and crude horseradish peroxidase
The gel is stained with DAB (diaminobenzidine) to ensure that the antibody does not inhibit the enzymatic activity of the peroxidase. Identity and purity of the antibody is established by immunoelectrophoresis. Electrophoresis of the antibody preparation followed by diffusion against anti-rabbit IgG and anti-rabbit whole serum results in single arcs of precipitation in the γ region
Each mL will precipitate a minimum of 0.25 mg of peroxidase at equivalence. May be used to stain axons and synaptic terminals in Drosophila embryo fillets.
The gel is stained with DAB (diaminobenzidine) to ensure that the antibody does not inhibit the enzymatic activity of the peroxidase. Identity and purity of the antibody is established by immunoelectrophoresis. Electrophoresis of the antibody preparation followed by diffusion against anti-rabbit IgG and anti-rabbit whole serum results in single arcs of precipitation in the γ region
Each mL will precipitate a minimum of 0.25 mg of peroxidase at equivalence. May be used to stain axons and synaptic terminals in Drosophila embryo fillets.
Inmunógeno
Purified peroxidase from horseradish
Aplicación
Anti-Peroxidase antibody produced in rabbit has been used in:
- immunoblot assays
- enzyme-linked-immunosorbent assay (ELISA)
- immunoprecipitation
Acciones bioquímicas o fisiológicas
Peroxidase helps in catalyzing the oxidation of a number of substrates with the help of hydrogen peroxide.
Forma física
Lyophilized from 0.01 M phosphate buffered saline, pH 7.2
Reconstitución
Reconstitute with deionized water.
Nota de análisis
Specificity is determined by immunoelectrophoresis using a crude peroxidase extract.
Cláusula de descargo de responsabilidad
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Código de clase de almacenamiento
11 - Combustible Solids
Clase de riesgo para el agua (WGK)
WGK 3
Punto de inflamabilidad (°F)
Not applicable
Punto de inflamabilidad (°C)
Not applicable
Equipo de protección personal
Eyeshields, Gloves, type N95 (US)
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