P7623

PKD2 Active human

recombinant, expressed in E. coli, ≥70% (SDS-PAGE), aqueous glycerol solution

• Sinónimos: Protein Kinase D2
• Número MDL: MFCD07371065
• Código UNSPSC: 12352200
• NACRES: NA.32

Propiedades

• recombinante: expressed in E. coli
• Nivel de calidad: 200
• Análisis: ≥70% (SDS-PAGE)
• formulario: aqueous glycerol solution
• actividad específica: 100 units/mg protein (Bradford)
• mol peso: protein apparent mol wt ~140 kDa
• Nº de acceso UniProt: Q9BZL6
• Condiciones de envío: dry ice
• temp. de almacenamiento: −70°C
• Información sobre el gen: human ... PRKD2(25865)

Descripción

Aplicación

Kinase activity is measured as the molar amount of phosphate incorporated into the CREBTIDE substrate peptide (KRREILSRRPSYR), per minute per mg protein at 30 °C using a final concentration of 50 μM [³²P] ATP.

Acciones bioquímicas o fisiológicas

PKD2 is a novel phorbol ester- and growth factor-stimulated serine/threonine kinase that contains two cysteine-rich motifs at the N terminus, a pleckstrin homology domain, and a catalytic domain. The PKD family of enzymes have been implicated in very diverse cellular functions, including Golgi organization and plasma membrane directed transport, metastasis, immune responses, apoptosis and cell proliferation.

Definición de unidad

One unit will incorporate one nanomole of phosphate into the CREBTIDE substrate (KRREILSRRPSYR) per minute at 30 °C at pH 7.2 using a final concentration of 50 μM [32P] ATP.

Forma física

Solution of 50 μl in 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 0.25 mM dithiothreitol (DTT), 0.1 mM EGTA, and 30% glycerol.

Información de seguridad

• Código de clase de almacenamiento: 10 - Combustible liquids