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P6248

Sigma-Aldrich

Anti-Parkin antibody, Mouse monoclonal

clone PRK8, purified from hybridoma cell culture

Sinónimos:

Anti-AR-JP, Anti-LPRS2, Anti-PARK2, Anti-PDJ

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About This Item

Número MDL:
MFCD02263896
Código UNSPSC:
12352203
NACRES:
NA.41

origen biológico

mouse

Nivel de calidad

200

conjugado

unconjugated

forma del anticuerpo

purified immunoglobulin

tipo de anticuerpo

primary antibodies

clon

PRK8, monoclonal

formulario

buffered aqueous solution

reactividad de especies

mouse, rat, hamster, human

concentración

~2 mg/mL

técnicas

microarray: suitable
western blot: 0.25-0.5 μg/mL using using rat brain cytosolic S1 extract

isotipo

IgG2b

Condiciones de envío

dry ice

temp. de almacenamiento

−20°C

modificación del objetivo postraduccional

unmodified

Información sobre el gen

human ... PARK2(5071)
mouse ... Park2(50873)
rat ... Park2(56816)

Descripción general

Monoclonal Anti- Parkin (mouse IgG2b isotype) is derived from the hybridoma PRK8 produced by the fusion of mouse myeloma cells (SP2 cells) and splenocytes from BALB/c mice immunized with recombinant human Parkin. Parkin is an ubiquitin-protein ligase expresses in many tissues such as brain, testis, skeletal muscle and heart. The parkin gene encodes a protein of 465 amino acid residues with moderate similarity to ubiquitin at the amino-terminus and a ring in between ring fingers (IBR)-ring-finger motif at the carboxyl-terminus. The gene has 12 exons.
Parkin is an ubiquitin-protein ligase expresses in many tissues such as brain, testis, skeletal muscle and heart. It is a RING-type E3 ubiquitin-protein ligase that facilitates the ubiquitination of misfolded proteins and protects from neurotoxic effect caused by these proteins. Monoclonal anti-parkin antibody can be used in microarray and immunoprecipitation assays. It can also be used in western blotting. Mouse anti-parkin antibody reacts specifically with parkin (50kD approx) of rat, hamster, mouse and human.

Inmunógeno

recombinant human Parkin.

Aplicación

Monoclonal anti-parkin antibody has been used in:
  • Enzyme linked immunosorbent assay (ELISA)
  • Immunoblotting
  • Immunoprecipitation[63}
  • Immunocytochemistry
  • to label parkin in pull-down assay

Forma física

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Código de clase de almacenamiento

10 - Combustible liquids

Clase de riesgo para el agua (WGK)

WGK 3

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable

Equipo de protección personal

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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Visite la Librería de documentos

Genomic organization and expression of parkin in Drosophila melanogaster
Bae JY, et al.
Experimental & Molecular Medicine, 35(5), 393-393 (2003)
Parkin regulates kainate receptors by interacting with the GluK2 subunit
Maraschi AM, et al,
Nature Communications, 5(46) (2014)
Victoriane Peugnet et al.
Antioxidants (Basel, Switzerland), 11(4) (2022-04-24)
Heart failure, mostly associated with cardiac hypertrophy, is a major cause of illness and death. Oxidative stress causes accumulation of reactive oxygen species (ROS), leading to mitochondrial dysfunction, suggesting that mitochondria-targeted therapies could be effective in this context. The purpose
Koji Yamano et al.
The Journal of biological chemistry, 290(42), 25199-25211 (2015-08-12)
Damaged mitochondria are eliminated through autophagy machinery. A cytosolic E3 ubiquitin ligase Parkin, a gene product mutated in familial Parkinsonism, is essential for this pathway. Recent progress has revealed that phosphorylation of both Parkin and ubiquitin at Ser(65) by PINK1
Jérôme Piquereau et al.
Autophagy, 9(11), 1837-1851 (2013-10-15)
Mitochondrial quality control plays a vital role in the maintenance of optimal mitochondrial function. However, its roles and regulation remain ill-defined in cardiac pathophysiology. Here, we tested the hypothesis that PARK2/Parkin, an E3-ligase recently described as being involved in the
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