P5747

Monoclonal Anti-Phosphoserine antibody produced in mouse

clone PSR-45, purified from hybridoma cell culture, buffered aqueous solution

• Sinónimos: Monoclonal Anti-Phosphoserine, Phospho Ser, Phospho serine, Phospho−Ser, Phospho−serine
• Código UNSPSC: 12352203
• NACRES: NA.41

Propiedades

• origen biológico: mouse
• Nivel de calidad: 200
• conjugado: unconjugated
• forma del anticuerpo: purified from hybridoma cell culture
• tipo de anticuerpo: primary antibodies
• clon: PSR-45, monoclonal
• Formulario: buffered aqueous solution
• envase: antibody small pack of 25 μL
• concentración: ~2 mg/mL
• técnicas: dot blot: suitable; indirect ELISA: 0.3-0.6 μg/mL using phosphoserine conjugated to BSA; microarray: suitable; western blot: 2.5-5.0 μg/mL using total rat brain extract.
• isotipo: IgG1
• Condiciones de envío: dry ice
• temp. de almacenamiento: −20°C
• modificación del objetivo postraduccional: unmodified

Descripción

Especificidad

By ELISA and dot blot, the antibody reacts specifically with phosphorylated serine, both as free amino acid or conjugated to carriers such as BSA or KLH. No cross-reactivity is observed with non-phosphorylated serine, phosphothreonine, phosphotyrosine, AMP or ATP. This antibody has been used in immunoblotting for the localization of some phosphoserine-containing proteins. Certain proteins known to contain phosphorylated serine may not be recognized by this antibody due to steric hindrance of the recognition site.

Inmunógeno

phosphoserine conjugated to Keyhole Limpet Hemocyanin (KLH).

Aplicación

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Western Blotting (1 paper)

The antibody has been used for the detection of some phosphoserine containing proteins using immunoblotting and dot blotting.

Acciones bioquímicas o fisiológicas

Protein phosphorylation and dephosphorylation are basic signaling mechanisms that modify protein function in eukaryotic cells. Phosphorylation is a rare posttranslational event in normal tissues, however, the abundance of phosphorylated cellular proteins increases several folds following various activation processes. The main amino acids that are phosphorylated are tyrosine, serine, or threonine (pTyr/pSer/pThr), each having specific kinases that phosphorylate them and specific phosphatases that dephosphorylate them. Mono and polyclonal antibodies directed against phosphorylated residues were generated and found useful as analytical and preparative tools by enabling the identification, quantification, and immunoaffinity isolation of phosphorylated cellular proteins.

Forma física

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Información de seguridad

• Código de clase de almacenamiento: 10 - Combustible liquids
• Clase de riesgo para el agua (WGK): nwg
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable