P4749
Anti-PADI4 (N-terminal) antibody produced in rabbit
IgG fraction of antiserum, buffered aqueous solution
Sinónimos:
Anti-PAD4, Anti-PADI5, Anti-PDI4, Anti-Peptidyl Arginine Deaminase Type IV, Anti-Peptidyl Arginine Deaminase V
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About This Item
Productos recomendados
origen biológico
rabbit
Nivel de calidad
conjugado
unconjugated
forma del anticuerpo
IgG fraction of antiserum
tipo de anticuerpo
primary antibodies
clon
polyclonal
formulario
buffered aqueous solution
mol peso
antigen 67 kDa
reactividad de especies
human
validación mejorada
recombinant expression
Learn more about Antibody Enhanced Validation
técnicas
immunoprecipitation (IP): 2-4 μL using DMSO-treated HL60 cells
microarray: suitable
western blot (chemiluminescent): 1:500-1:1,000 using DMSO-treated HL60 cells
Nº de acceso UniProt
Condiciones de envío
dry ice
temp. de almacenamiento
−20°C
modificación del objetivo postraduccional
unmodified
Información sobre el gen
human ... PADI4(23569)
Descripción general
PAD4 (also designated PADI4 or PAD type IV), one of the members of the PAD family. PAD4 was initially named PAD5. However, since it is closely related to rodent PAD4 it was renamed human PAD4. The most noticeable difference between the isotypes is their tissue-specific expression. Expression of the PAD4 gene is tightly linked to myeloid differentiation. It is expressed in granulocytes and monocytes and expressed in the nucleus.
Peptidyl arginine deiminase 4 (PADI4) is one of the four PADIs found in humans. The protein consists of 663 amino acids. PADI4 gene is localized to human chromosome 1p36. It is expressed in hematopoietic tissues, such as spleen, thymus, peripheral blood leucocytes, fetal liver and bone marrow.
Inmunógeno
synthetic peptide corresponding to amino acids 1-15 of human PADI4, conjugated to KLH via a C-terminal added cysteine residue. The immunizing peptide is specific to human.
Aplicación
Anti-PADI4 (N-terminal) antibody produced in rabbit has been used in immunoblotting and immunoprecipitation.
Anti-PADI4 (N-terminal) antibody produced in rabbit has been used in immunohistochemistry and immunofluorescence.
Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Chromatin immunoprecipitation (1 paper)
Chromatin immunoprecipitation (1 paper)
Acciones bioquímicas o fisiológicas
PADs (also known as PADIs), peptidylarginine deiminases, are a family of enzymes, which convert protein-bound arginine residues to citrulline residues in the presence of calcium ions, a process termed deimination. Among the 5 isoforms of PADs, only PAD4 has been identified as an RA-susceptibility gene.
Peptidyl arginine deiminase 4 (PADI4) is responsible for the post-translational conversion of peptidylarginine to citrulline, in the presence of calcium ions. In individuals with rheumatoid arthritis (RA), this gene is expressed in hematological cells and synovial tissues, and variant in this gene is linked with susceptibility to RA. The expression of this protein is linked with DNA hypermethylation in acute promyelocytic leukemia (APL).
Forma física
Solution in 0.01 M phosphate buffered saline, pH 7.4, and 15 mM sodium azide.
Cláusula de descargo de responsabilidad
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Referencia del producto
Descripción
Precios
Código de clase de almacenamiento
10 - Combustible liquids
Clase de riesgo para el agua (WGK)
WGK 3
Punto de inflamabilidad (°F)
Not applicable
Punto de inflamabilidad (°C)
Not applicable
Certificados de análisis (COA)
Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»
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Encuentre la documentación para los productos que ha comprado recientemente en la Biblioteca de documentos.
Potential Role for PAD2 in Gene Regulation in Breast Cancer Cells
Brian D
PLoS ONE (2012)
Melissa M Ledet et al.
BMC cancer, 18(1), 412-412 (2018-04-14)
Mammary cancer is highly prevalent in dogs and cats and results in a poor prognosis due to critically lacking viable treatment options. Recent human and mouse studies have suggested that inhibiting peptidyl arginine deiminase enzymes (PAD) may be a novel
Protein arginine deiminase 4 (PAD4): current understanding and future therapeutic potential
Jones J, et al.
Current Opinion in Drug Discovery & Development, 12(5), 616-616 (2009)
Coleman H Young et al.
Reproduction (Cambridge, England), 153(1), 1-10 (2017-01-01)
There are five peptidylarginine deiminase (PAD) isozymes designated as PADs 1, 2, 3, 4 and 6, and many are expressed in female reproductive tissues. These enzymes post-translationally convert positively charged arginine amino acids into neutral citrulline residues. Targets for PAD-catalyzed
Sunish Mohanan et al.
Frontiers in immunology, 4, 67-67 (2013-03-20)
PAD4-mediated hypercitrullination of histone H4 arginine 3 (H4R3) has been previously found to promote the formation of Neutrophil Extracellular Traps in inflamed tissues and the resulting histone H4 citrulline 3 (H4Cit3) modification is thought to play a key role in
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