P3326

Platelet-Derived Growth Factor-AB human

≥97% (SDS-PAGE and N-terminal analysis), recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture

• Sinónimos: PDGF-AB
• Número MDL: MFCD00213024
• Código UNSPSC: 12352202
• NACRES: NA.77

Propiedades

• product name: Platelet-Derived Growth Factor-AB human, PDGF-AB, recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture
• origen biológico: human
• Nivel de calidad: 200
• recombinante: expressed in E. coli
• Análisis: ≥97% (SDS-PAGE and N-terminal analysis)
• formulario: lyophilized powder
• potencia: 10-100 ng/mL ED₅₀/EC₅₀
• calidad: endotoxin tested
• mol peso: 27-31 kDa
• envase: pkg of 10 μg; pkg of 50 μg
• condiciones de almacenamiento: avoid repeated freeze/thaw cycles
• técnicas: cell culture | mammalian: suitable
• impurezas: ≤1 EU/μg
• Nº de acceso UniProt: P04085, P01127
• temp. de almacenamiento: −20°C
• Información sobre el gen: human ... PDGFA(5154) , PDGFB(5155)

Descripción

Descripción general

Platelet-Derived Growth Factor (PDGF) is a cationic polypeptide and a constituent of α-granules. PDGF is composed of chain A and B linked together by a disulphide bridge. PDGF heterodimers occurs as PDGF-AB and homodimers exists as FDGF-AA, PDGF-BB, PDGF-CC and PDGF-DD). The PDGFB gene is localized to pericytes in the brain and is mapped to human chromosome 7p22.3.

Aplicación

Platelet-Derived Growth Factor-AB human has been used in luciferase reporter assay.

Acciones bioquímicas o fisiológicas

Cellular actions of platelet-derived growth factor (PDGF) include chemoattraction and activation of neutrophils, monocytes, and fibroblasts. PDGF appears to play a crucial role in the cellular response to tissue injury and is critically involved in the pathological progression of atherosclerosis.
PDGF stimulates fibroblast proliferation and extracellular matrix production in order to facilitate tissue repair. PDGF-AB isomer is found to be associated with angiogenesis and proliferation. Loss of PDGFB gene function induces microvascular calcification due to age-dependent phenotypical changes.

Otras notas

Heterodimer of the A and B chains of PDGF disulfide linked.

Forma física

Lyophilized from a 0.2 μm filtered solution in 40% acetonitrile and 0.1% trifluoroacetic acid.

Nota de análisis

The biological activity is measured by its ability to stimulate ³H-thymidine incorporation in quiescent NR6R-3T3 fibroblasts.

Información de seguridad

• Código de clase de almacenamiento: 11 - Combustible Solids
• Clase de riesgo para el agua (WGK): WGK 3
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable
• Equipo de protección personal: Eyeshields, Gloves, type N95 (US)