P2482

Monoclonal Anti-Protein Kinase Bα antibody produced in mouse

clone PKB-175, ascites fluid

• Sinónimos: Anti-Akt1, Anti-PKBα
• Número MDL: MFCD01634055
• Código UNSPSC: 12352203
• NACRES: NA.44

Propiedades

• origen biológico: mouse
• conjugado: unconjugated
• forma del anticuerpo: ascites fluid
• tipo de anticuerpo: primary antibodies
• clon: PKB-175, monoclonal
• mol peso: antigen 56 kDa
• contiene: 15 mM sodium azide
• reactividad de especies: chicken, human, bovine, rat, mouse
• técnicas: indirect ELISA: suitable; microarray: suitable; western blot: 1:5,000 using human MCF-7 cell extract
• isotipo: IgG1
• Nº de acceso UniProt: P31749
• Condiciones de envío: dry ice
• temp. de almacenamiento: −20°C
• modificación del objetivo postraduccional: unmodified
• Información sobre el gen: human ... AKT1(207) ; mouse ... Akt1(11651) ; rat ... Akt1(24185)

Descripción

Descripción general

Monoclonal Anti-Protein Kinase Bα (PKBα/Akt1) (mouse IgG1 isotype) is derived from the PKB-175 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from a BALB/c mouse. PKB/Akt is composed of an N-terminal pleckstrin-homology (PH) domain, followed by a catalytic kinase domain and a short C-terminal regulatory domain. This cytoplasmic protein is made up of 480 amino acids. AKT1 is mapped to human chromosome 14q32.

Protein Kinase B (also known as Akt, or RAC-PK) is a protein that belongs to serine/threonine kinases.

Especificidad

Monoclonal Anti-Protein Kinase Bα (PKBα/Akt1) recognizes PKB isoform α.

Inmunógeno

Synthetic peptide corresponding to amino acids 461-477 of human PKBα/Akt1, conjugated to KLH. The sequence is identical in mouse, rat, and bovine PKBα/Akt1, highly conserved in PKBβ/Akt2, and diverges in PKBγ.

Aplicación

Monoclonal Anti-Protein Kinase Bα antibody produced in mouse has been used in:

• western blot
• immunofluorescence
• enzyme-linked immunosorbent assay (ELISA)

Acciones bioquímicas o fisiológicas

Protein kinase Bα (PKBα/Akt1) plays a crucial role as a suppressor of apoptotic cell death in different cell types, induced by a variety of stimuli including growth factor withdrawal, loss of cell adhesion and DNA damage. It can protect cerebellar neurons from apoptosis induced by insulin-like growth factor 1 (IGF-1) withdrawal. Akt is the cellular homologue of the viral oncogene v-akt of the AKT-8 acute transforming retrovirus found in rodent T cell lymphoma. It is considered to play an important role in the control of cell cycle, cell proliferation and differentiation . AKT plays a key role in angiogenesis.

Forma física

Monoclonal Anti-Protein Kinase Bα (PKBα/Akt1) is supplied as ascites fluid containing 15 mM sodium azide

Almacenamiento y estabilidad

For continuous use, store at 2-8C for upto one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing is not recommended. Storage in "frost-free" freezers is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12hours.

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Información de seguridad

• Código de clase de almacenamiento: 12 - Non Combustible Liquids
• Clase de riesgo para el agua (WGK): nwg
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable