P0076

Anti-PINK1 antibody produced in rabbit

~1.5 mg/mL, affinity isolated antibody, buffered aqueous solution

• Sinónimos: Anti-BRPK, Anti-PARK6, Anti-PTEN-induced putative kinase protein 1, Anti-Serine/threonine-protein kinase PINK1, mitochondrial precursor
• Número MDL: MFCD08064431
• Código UNSPSC: 12352203
• NACRES: NA.41

Propiedades

• origen biológico: rabbit
• conjugado: unconjugated
• forma del anticuerpo: affinity isolated antibody
• tipo de anticuerpo: primary antibodies
• clon: polyclonal
• formulario: buffered aqueous solution
• mol peso: antigen ~60 kDa
• reactividad de especies: mouse (predicted), human, rat (predicted)
• validación mejorada: recombinant expression; Learn more about Antibody Enhanced Validation
• concentración: ~1.5 mg/mL
• técnicas: western blot: 1-2 μg/mL using HEK-293T cell lysate expressign human PINK1
• Nº de acceso UniProt: Q9BXM7
• Condiciones de envío: dry ice
• temp. de almacenamiento: −20°C
• modificación del objetivo postraduccional: unmodified
• Información sobre el gen: human ... PINK1(65018) ; mouse ... Pink1(68943) ; rat ... Pink1(298575)

Descripción

Descripción general

PTEN induced putative kinase 1 (PINK1) is a serine/threonine kinase expressed in mitochondria. It contains an N-terminal mitochondrial targeting motif and a highly conserved kinase domain homologous to serine/threonine kinases of the Ca2+/calmodulin family.

Aplicación

Anti-PINK1 antibody produced in rabbit has been used in immunoblotting.

Acciones bioquímicas o fisiológicas

PINK1 (PTEN induced putative kinase 1, also known as PARK6 and BRPK), has been identified as linked to the autosomal recessive form of familial Parkinson disease (PD). PINK1 localized to the mitochondria protects cells from stress-induced mitochondrial dysfunction. Overexpression of wild-type PINK1 has been found to protect neurons from stress-induced mitochondrial dysfunction and apoptosis. Genetic studies in drosophila indicate that PINK1 acts upstream of Parkin in a common pathway that influences mitochondrial morphology. PINK1 activity has been shown to protect primary neurons in mouse from the dopaminergic neurotoxin MPTP (1-methyl-4-phenylpyridine (MPP+)/1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine) both in vitro and in vivo. This protective activity requires PINK1 kinase activity, as a PINK1 G309D mutant linked to familial PD or a kinase dead mutant K219M are not protective. PINK1 has been shown to be cleaved and localized to the mitochondria, in response to enhanced proteasomal stress in vitro and correlates with increased expression of the processed PINK1 protein in PD brain.

Descripción de destino

PINK1 is a Ser/Thr kinase that has beenlocalized to the mitochondria, and thought to protectcells from stress-induced mitochondrial dysfunction.

Forma física

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Información de seguridad

• Código de clase de almacenamiento: 10 - Combustible liquids
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable
• Equipo de protección personal: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)