OGS3213
PSF-CMV-PURO-NH2-FLAG® - N-TERMINAL FLAG® TAG MAMMALIAN PLASMID
plasmid vector for molecular cloning
Sinónimos:
cloning vector, expression vector, molecular cloning vector, plasmid, plasmid vector, snapfast vector, vector
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About This Item
Código UNSPSC:
12352200
NACRES:
NA.85
Productos recomendados
etiqueta
FLAG® tagged
formulario
buffered aqueous solution
mol peso
size 6173 bp
selección de bacterias
kanamycin
selección de células de mamífero
puromycin
Origen de replicación
pUC (500 copies)
Escisión peptídica
no cleavage
Ubicación de la etiqueta en el péptido
N-terminal
Promotor
Promoter name: CMV
Promoter activity: constitutive
Promoter type: mammalian
gen reportero
none
Condiciones de envío
ambient
temp. de almacenamiento
−20°C
Descripción general
This plasmid is designed to express tagged proteins in mammalian cells either by transient transfection or by creating stable cell lines. It contains a puromycin resistance expression cassette using the human Ubiquitin promoter to drive expression and allow for the selection of cells containing the plasmid.
About the Peptide Tag:This plasmid contains an n-terminal FLAG epitope tag that can be fused to a gene of interest to allow protein detection and/or purification. The sequence of the tag is: DYKDDDDK.
Promoter Expression Level: This plasmid contains the mammalian CMV promoter to drive gene expression. We have tested all of our mammalian promoters in a range of cell types and CMV is consistently the strongest in those we have studied. However there are many reports of the CMV promoter demonstrating silencing by methylation in long-term culture.
About the Peptide Tag:This plasmid contains an n-terminal FLAG epitope tag that can be fused to a gene of interest to allow protein detection and/or purification. The sequence of the tag is: DYKDDDDK.
Promoter Expression Level: This plasmid contains the mammalian CMV promoter to drive gene expression. We have tested all of our mammalian promoters in a range of cell types and CMV is consistently the strongest in those we have studied. However there are many reports of the CMV promoter demonstrating silencing by methylation in long-term culture.
Secuencia
To view sequence information for this product, please visit the product page
Nota de análisis
To view the Certificate of Analysis for this product, please visit www.oxgene.com
Información legal
FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany
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Referencia del producto
Descripción
Precios
Código de clase de almacenamiento
12 - Non Combustible Liquids
Punto de inflamabilidad (°F)
Not applicable
Punto de inflamabilidad (°C)
Not applicable
Certificados de análisis (COA)
Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»
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