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OGS3211

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PSF-CMV-PURO-NH2-HIS6 - N-TERMINAL 6 HIS TAG MAMMALIAN PLASMID

plasmid vector for molecular cloning

Sinónimos:

cloning vector, expression vector, molecular cloning vector, plasmid, plasmid vector, snapfast vector, vector

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About This Item

Código UNSPSC:
12352200
NACRES:
NA.85

etiqueta

6-His tagged

formulario

buffered aqueous solution

mol peso

size 6167 bp

selección de bacterias

kanamycin

selección de células de mamífero

puromycin

Origen de replicación

pUC (500 copies)

Escisión peptídica

no cleavage

Ubicación de la etiqueta en el péptido

N-terminal

Promotor

Promoter name: CMV
Promoter activity: constitutive
Promoter type: mammalian

gen reportero

none

Condiciones de envío

ambient

temp. de almacenamiento

−20°C

Descripción general

This plasmid is designed to express tagged proteins in mammalian cells either by transient transfection or by creating stable cell lines. It contains a puromycin resistance expression cassette using the human Ubiquitin promoter to drive expression and allow for the selection of cells containing the plasmid.

About the Peptide Tag:This plasmid contains an n-terminal Hexa-Histidine (6His) affinity tag that can be fused to a gene of interest to allow protein detection and/or purification. The sequence of the tag is: HHHHHH.

Promoter Expression Level: This plasmid contains the mammalian CMV promoter to drive gene expression. We have tested all of our mammalian promoters in a range of cell types and CMV is consistently the strongest in those we have studied. However there are many reports of the CMV promoter demonstrating silencing by methylation in long-term culture.

Secuencia

To view sequence information for this product, please visit the product page

Nota de análisis

To view the Certificate of Analysis for this product, please visit www.oxgene.com

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Descripción
Precios

Código de clase de almacenamiento

12 - Non Combustible Liquids

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable


Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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