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OGS3162

Sigma-Aldrich

PSF-OXB20-NH2-PHOA - PHOA SECRETION PLASMID

plasmid vector for molecular cloning

Sinónimos:

cloning vector, expression vector, molecular cloning vector, plasmid, plasmid vector, snapfast vector, vector

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About This Item

Código UNSPSC:
12352200

recombinante

expressed in E. coli

formulario

buffered aqueous solution

mol peso

size 3911 bp

selección de bacterias

kanamycin

Origen de replicación

pUC (500 copies)

Escisión peptídica

no cleavage

Ubicación de la etiqueta en el péptido

N-terminal

Promotor

Promoter name: OXB20
Promoter activity: constitutive
Promoter type: bacterial

gen reportero

none

Señal de secreción

PhoA

Condiciones de envío

ambient

temp. de almacenamiento

−20°C

Descripción general

This plasmid is designed to express tagged proteins in E. coli. The plasmid contains a constitutive promoter (OXB20) derived from the region upstream of the E. coli RecA gene. It does not require induction or any additional components for activity. It is the strongest of the bacterial promoters that we provide and this high level of expression can cause expression problems with some proteins with poor solubility. For this reason we sell a range of bacterial promoters with different expression levels (OXB1(low)>OXB20(high)) that can be provided with the peptide tags in this plasmid on request.

About the Peptide Tag:This plasmid contains an PhoA secretory signal peptide (SP) to allow proteins to be exported from the cytosol. During translocation from the cytosol the signal peptide is removed from the protein by endogenous proteases. .

Promoter Expression Level: This plasmid contains a constitutive bacterial promoter that does not require induction. It is the strongest bacterial promoter we sell and this can cause solubility and expression problems with some proteins. We also offer a range of other bacterial promoters that are compatible with this plasmid and are available on request.

Secuencia

To view sequence information for this product, please visit the product page

Nota de análisis

To view the Certificate of Analysis for this product, please visit www.oxgene.com

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Precios

Código de clase de almacenamiento

12 - Non Combustible Liquids

Punto de inflamabilidad (°C)

Not applicable


Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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