O3514

Bovine Osteopontin

from bovine milk, powder, suitable for cell culture

• Sinónimos: Bone Sialoprotein-1, Early T Lymphocyte Activation Protein-1 (ETA-1), OPN, Secreted Phosphoprotein 1 (Spp1)
• Número MDL: MFCD03100698
• Código UNSPSC: 12352202
• NACRES: NA.32

Propiedades

• product name: Osteopontin from bovine milk, lyophilized powder
• origen biológico: bovine milk
• Nivel de calidad: 100
• esterilidad: sterile
• formulario: lyophilized powder
• mol peso: ~60 kDa
• envase: pkg of 50 μg
• técnicas: cell culture | mammalian: suitable
• Nº de acceso UniProt: P31098; P31096
• Condiciones de envío: ambient
• temp. de almacenamiento: −20°C
• Información sobre el gen: cow ... SPP1(281499)

Descripción

Descripción general

Osteopontin (OPN) is a multifunctional protein. It is released by macrophages, T cells and epithelial cells. It has 261 amino acid residues with a molecular mass of 29,283 Da. Osteopontin is also called as uropontin. Osteopontin from bovine milk is highly glycosylated and phosphorylated. SPP1 gene (secreted phosphoprotein 1) codes for osteopontin. This gene is located on human chromosome 4 and cow chromosome 6. It has seven exons and six introns.

Acciones bioquímicas o fisiológicas

A secreted, highly acidic, calcium binding, phosphorylated glycoprotein that is chemotactic for macrophages, smooth muscle cells, endothelial cells, and glial cells. In activated macrophages, OPN inhibits nitric oxide production and cytotoxicity. Human, mouse, rat, pig, and bovine OPN share 40% amino acid sequence identity.

Osteopontin stimulates cell mediated responses, chemotaxis of inflammatory cells and anti-inflammatory responses. It binds to av-containing integrins and stimulate cell adhesion and migration. Osteopontin promotes Th1 type immunity.

Forma física

Lyophilized from a 0.2 μm filtered solution in phosphate buffered saline containing 2.5 mg bovine serum albumin.

Nota de análisis

The biological activity is measured by its ability to mediate 293 cell adhesion.

Información de seguridad

• Código de clase de almacenamiento: 11 - Combustible Solids
• Clase de riesgo para el agua (WGK): WGK 3
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable
• Equipo de protección personal: Eyeshields, Gloves, type N95 (US)