N7885

Neuraminidase from Vibrio cholerae

Type III, buffered aqueous solution, 0.2 μm filtered, 1-5 units/mg protein (Lowry, using NAN-lactose)

• Sinónimos: Acyl-neuraminyl Hydrolase, Receptor-destroying enzyme, Sialidase
• Número de CAS: 9001-67-6
• Comisión internacional de enzimas: 3.2.1.18 (BRENDA, IUBMB)
• Número CE: 232-624-6
• Número MDL: MFCD00131711
• Código UNSPSC: 12352204
• NACRES: NA.54

Propiedades

• Nivel de calidad: 300
• tpo: Type III
• formulario: buffered aqueous solution
• actividad específica: 1-5 units/mg protein (Lowry, using NAN-lactose)
• mol peso: 83 kDa
• aplicaciones: diagnostic assay manufacturing
• actividad extraña: Protease and NAN-aldolase, present
• temp. de almacenamiento: 2-8°C

Descripción

Descripción general

Neuraminidase enzymes are glycoside hydrolase enzymes that catalyze hydrolysis of terminal sialic acid residues. The most well-known are the viral nearamidases, which promote influenza virus release.

Aplicación

Neuraminidase from Vibrio cholerae has been used in a study to assess its role in the binding and uptake of cholera toxin by susceptible cells. It has also been used in a study to investigate the preparation of a sodium substrate and its use in a fluorometric assay of neuraminidase.

Neurminidase is used as a cell-surface probe for glycoconjugate distribution and in substrate specificity studies.

Acciones bioquímicas o fisiológicas

Vibrio cholerae neuraminidase has been shown to promote cholera toxin infection by binding the toxin and aiding in its uptake by cells.

Calidad

Preservative free.

Definición de unidad

One unit will liberate 1.0 μmole of N-acetylneuraminic acid per min at pH 5.0 at 37 °C using NAN-lactose or bovine submaxillary mucin, unless otherwise specified. Prices based on units using NAN-lactose as substrate.

Forma física

Aqueous solution, pH 5.5, containing 0.15 M NaCl and 4 mM CaCl₂.

Nota de preparación

Chromatographically purified.

Información de seguridad

• Pictogramas: GHS08
• Palabra de señalización: Danger
• Frases de peligro: H334
• Consejos de prudencia: P261 - P284 - P501
• Clasificaciones de peligro: Resp. Sens. 1
• Código de clase de almacenamiento: 10 - Combustible liquids
• Clase de riesgo para el agua (WGK): WGK 1
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable
• Equipo de protección personal: Eyeshields, Gloves, type N95 (US)