N4662

Nek6, Active human

recombinant, expressed in E. coli, ≥80% (SDS-PAGE), buffered aqueous glycerol solution

• Sinónimos: Gene A-Related Kinase 6, NIMA-Related Kinase 6, Never in Mitosis
• Número MDL: MFCD07370972
• Código UNSPSC: 12352200
• NACRES: NA.32

Propiedades

• recombinante: expressed in E. coli
• Nivel de calidad: 100
• Ensayo: ≥80% (SDS-PAGE)
• Formulario: buffered aqueous glycerol solution
• enzyme activity: ≥10 units/mg protein
• mol peso: protein apparent mol wt ~58 kDa
• Nº de acceso UniProt: Q9HC98
• Condiciones de envío: dry ice
• temp. de almacenamiento: −70°C
• Información sobre el gen: human ... NEK6(10783)

Descripción

Aplicación

Kinase activity is measured as the molar amount of phosphate incorporated into myelin basic protein, per minute per mg protein at 30 °C using a final concentration of 50 μM [³²P] ATP.

Acciones bioquímicas o fisiológicas

Nek6 is a nuclear and cytoplasmic kinase that is required for mitotic progression of human cells. It is phosphorylated and activated during M phase of the cell cycle. Inhibition of Nek6 arrests cells in M phase and triggers apoptosis, suggesting that Nek6 is required for metaphase-anaphase transition.

Definición de unidad

One unit will incorporate 1 nanomole of phosphate into myelin basic protein (MBP) per minute at pH 7.2 at 30 deg C using a final concentration of 50 μm (32p) ATP

Forma física

Solution in 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 0.25 mM DTT, 0.1 mM EGTA, and 30% glycerol.

Información de seguridad

• Código de clase de almacenamiento: 12 - Non Combustible Liquids
• Clase de riesgo para el agua (WGK): WGK 1
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable